目的 探究天蟾胶囊的外周和中枢系统镇痛效应差异。方法 首先采用甩尾方法将基础痛阈差距较小的64只C57BL/6雄性小鼠随机分为对照组、天蟾组、天蟾纳洛酮腹腔组、天蟾纳洛酮脊髓组、天蟾纳洛酮脑室组、天蟾β-氟硝胺（β-fluorine nitramine，β-FNA）组、天蟾纳曲多尔组和天蟾诺比萘哌嗪组，除对照组外，其余各组分别ig 42.60 g/kg天蟾胶囊，连续给药1周，给药结束后，各组分别以不同给药途径给予各阻断剂，30、60、90、120、150和180 min后观察小鼠的痛阈变化。结果 给予天蟾胶囊后，小鼠疼痛阈值升高，镇痛起效时间为给药后30 min（P＜0.001），作用峰值为给药后120 min（P＜0.05）。天蟾纳洛酮腹腔组给药后30、60 min，小鼠痛阈值明显高于天蟾胶囊脊髓和脑室给药组（P＜0.05），ip μ阿片受体拮抗剂的小鼠给药30、60 min痛阈值显著高于注射δ、κ阿片受体拮抗剂（P＜0.05）。结论 天蟾胶囊具有较好的镇痛作用，其镇痛效应对外周的阿片系统依赖程度高于中枢阿片系统，其作用的受体主要是外周μ阿片受体。

To investigate the difference of analgesic effect between peripheral and central system of Tianchan Capsule (天蟾胶囊). Methods First, 64 male C57BL/6 mice without significant basal pain threshold difference by tail flapping method were randomly divided into control group, Tianchan group, Tianchan naloxone intraperitoneal group, Tianchan naloxone spinal cord group, Tianchan naloxone ventricle group, Tianchan β-fluorine nitramine (β-FNA) group, Tianchan nattrel group and Tianchan norbinazine group. Except the control group, the other groups were administered Tianchan Capsule dose of 42.60 g/kg by gavage for one week. After the one week administration of Tianchan Capsule, each group was given blockers by different administration routes, and the changes of pain threshold were observed 30, 60, 90, 120, 150 and 180 min later. Results After administration of Tianchan Capsule, the pain threshold of mice was increased. The onset time of analgesia was 30 minutes after administration (P < 0.001) and the peak time was 120 min after administration (P < 0.05). At 30 and 60 min after intraperitoneal administration, the pain threshold of mice in Tianchan naloxone intraperitoneal group was significantly higher than that in Tianchan naloxone spinal cord group and Tianchan naloxone ventricle group (P < 0.05). The pain threshold of mice treated with μ opioid receptor antagonist at 30 and 60 min was significantly higher than that of mice injected with δ and κ opioid receptor antagonist (P < 0.05). Conclusion The analgesic effect of Tianchan Capsule in peripheral opioid system is better than that of central opioid system. It mainly targets μ opioid receptor.

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