目的 建立丹膝颗粒组成药味的UPLC指纹图谱，并运用总量统计矩、信息熵和信息量及1次投料量数学模型，计算出稳态1次投料量，建立动态中药成分群的稳态性质量提取方法。方法 采用UPLC法，色谱柱为Acquity UPLC HSS T3（100 mm×2.1 mm，1.8 μm）；流动相为乙腈-0.1%磷酸水溶液，梯度洗脱；体积流量为0.4 mL/min；240 nm UV检测；柱温为30 ℃；进样量为3 μL。建立丹参、牛膝、川芎等12味丹膝颗粒组成药味的UPLC指纹图谱，采用总量统计矩、信息熵和信息量分析方法获得指纹图谱的动态性参数，并根据遗传统计学原理获得单味药材及全方的1次投料量，指导工业化大生产，获得成分稳态的丹膝颗粒。结果 丹膝颗粒12味组成药味的平均总量统计矩零、一、二阶矩、信息熵、信息量分别为6.56×10⁴、13.36、50.71、5.75、3.59×10⁵；其中以桑寄生药材信息量的RSD最大，以川芎药材的RSD最小，平均为24.51%；按单味药材投料，据全方累积信息量的RSD计算出1次投料量为2.13 kg，按全方的比例投料，全方1次稳态投料量为335.35 kg，远远大于《中国药典》2020年版规定。结论 丹膝颗粒组成药味的指纹图谱动态性可用总量统计矩、信息熵、信息量进行分析，据此可获得稳态的一次投料量，据此投料生产可保证丹膝颗粒质量的稳定。

Objective To establish a UPLC fingerprint of composition of function ingredients of Danxi Granules (丹膝颗粒), and use total statistical moments, information entropy, information amount and one-time steady-state feeding quantity mathematical model, to calculate the one-time steady-state feeding quantity, and establish a steady-state quality extraction method for dynamic Chinese medicine component groups. Method UPLC method was adopted. The sample was separated on a chromatographic column of Acquity UPLC HSS T3 (100 mm × 2.1 mm, 1.8 μm) with acetonitrile-0.1% phosphoric acid solution as mobile phase, gradient elution at a volume flow rate of 0.4 mL/min, 240 nm UV detection; The column temperature was 30 ℃; The injection volume was 3 μL. UPLC fingerprint of 12 Chinese medicine herbs of composition of function ingredients of Danxi Granules was established. The dynamic parameters of the fingerprints were obtained by total statistical moment, information entropy and information amount. The one-time steady-state feeding quantity of single-medicinal herb and complex preparation were obtained according to the principles of genetic statistics, which guided industrial production and obtained Danxi Granules with stable ingredients. Results The average total statistical zero, first and second moments, information entropy, and information amount of the 12 composition of function ingredients of Danxi Granules were 6.56 × 10⁴, 13.36, 50.71, 5.75, 3.59 × 10⁵ respectively; Among them, the RSD of information amount of Chinese medicine herbs Taxillus chinensis was the largest one, and the RSD of Chuanxiong Rhizoma was the smallest one, with an average of 24.51%. According to single medicinal herb, the one-time steady-state feeding quantity calculated according to the RSD change of the accumulated information amount of the whole prescription was 2.13 kg. When one-time steady-state feeding quantity was 335.35 kg according to ratio of the whole prescription, which was far greater than that stipulated in Chinese Pharmacopoeia. Conclusion The dynamics of the fingerprint of composition of function ingredients Danxi Granule can be analyzed by total statistical moment, information entropy, and information amount. According to this, one-time steady-state feeding quantity can be obtained, and stability of the quality of Danxi Granules can be guaranteed.

R283.6

国家自然科学基金（81874507）；国家自然科学基金（81803729）；湖南省自然科学基金项目（2021JJ80058）；湖南省中医药科研项目（2021078）；长沙市科技计划项目（kq1907137）；湖南省研究生科研创新项目（CX20210689）；湖南中医药大学校级科研基金（2019XJJJ024）