目的 研究木荚红豆叶Ormosia xylocarpa 70%乙醇提取物的醋酸乙酯萃取物化学成分及其对肝癌HepG2的细胞毒性作用。方法 利用多种色谱学方法和波谱学技术分离鉴定化合物，采用CCK-8比色法测定化合物的细胞毒活性。结果 从木荚红豆叶70%乙醇提取物的醋酸乙酯萃取物中分离鉴定8个化合物，分别为3',4"-二羟基-3,5,4',3",5"-五甲氧基-7,9';7',9-双环氧-4,8"-氧-8,8'-倍半木脂素-丙醇（1）、染料木素（2）、槲皮素（3）、羽扇豆酮（4）、β-香树脂醇（5）、蒲公英赛醇（6）、mundulin（7）和齐墩果酸（8）。化合物7对人肝癌HepG2细胞有细胞毒性作用，半数抑制浓度（median inhibition concentration，IC₅₀）值为86.34 μmol/L。结论 所有化合物均首次从该植物中分离得到，其中化合物1为新的四氢呋喃类木脂素，命名为木荚红豆素；化合物5～8为首次从红豆属中分离得到，化合物7具有一定的细胞毒活性。

Objective To study the chemical constituents from the ethyl acetate fraction of the 70% ethanol extract from the leaves of Ormosia xylocarpa and their cytotoxic activities. Methods The compounds were separated by a variety of chromatographic methods and identified by spectroscopy techniques. The cytotoxic activities were tested by CCK-8 assay. Results Eight compounds were isolated from the ethyl acetate fraction of the 70% ethanol extract from the leaves of O. xylocarpa, which were identified as 3',4"-dihydroxy-3,5,4',3",5"-pentamethoxy-7,9';7',9-diepoxy-4,8"-oxy-8,8'-sesquineo-lignan-propanol (1), genistein (2), quercetin (3), lupinone (4), β-anulanol (5), epitaraxerol (6), mundulin (7), and oleanolic acid (8). Compound 7 showed moderate inhibition on HepG2 cells, with a IC₅₀ value of 86.34 μmol/L. Conclusion All compounds are obtained from this plant for the first time, among them, compound 1 is a new lignan, namely xylocarpalignan; Compounds 5—8 are obtained from the Ormosia for the first time. Compound 7 shows moderate inhibition on HepG2 cells.

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福建农林大学乡村振兴服务团队项目（11899170128）；全国农业教指委研究课题（2021-NYYB-12）；福建农林大学科技创新专项基金项目（KHF190014）