目的 建立虎杖 Polygonum cuspidatum 的 HPLC 指纹图谱，研究虎杖醇提物的 HPLC 指纹图谱与抗痛风性关节炎活性之间的谱效关系，初步明确虎杖抗痛风性关节炎主要活性成分。方法 采用 HPLC 法建立虎杖的指纹图谱；使用"中药色谱指纹图谱相似度指纹评价系统（2004 A 版）"进行相似度评价；以小鼠足垫厚度、血清尿酸含量为虎杖抗痛风性关节炎作用药效指标，采用灰色关联度分析法分析其谱效关系。结果 虎杖发挥抗痛风性关节炎作用是多种成分共同作用的结果，对抗痛风性关节炎作用贡献较大的特征峰有 7 号峰（虎杖苷）、9 号峰（槲皮素）、11 号峰（白藜芦醇）、12 号峰（槲皮苷）、13 号峰（大黄素-8-O-β-D-葡萄糖苷）、16 号峰（大黄素）、18 号峰（大黄素甲醚）。结论 虎杖发挥抗痛风性关节炎作用的药效是多种成分共同作用的结果， 可为该药材质量控制与应用开发提供参考。

To establish the HPLC fingerprint of Polygonum cuspidatum and to study the relationship between the HPLC fingerprint of P. cuspidatum alcohol extract and its anti gouty arthritis activity. Methods HPLC method was used to establish the fingerprint of P. cuspidatum; The "Fingerprint Similarity Evaluation System of Traditional Chinese Medicine Chromatogram (2004 A version)" was used to evaluate the similarity; The thickness of mouse foot pad and the content of serum uric acid were used as the pharmacodynamic indexes of P. cuspidatum in anti gouty arthritis, and the relationship between the spectrum and effect was analyzed by grey correlation analysis. ResultsThe anti gouty arthritis effect of P. cuspidatum was the result of the joint action of many components. The characteristic peaks that contributed a lot to the anti gouty arthritis effect were No.7 peak (polydatin), No.9 peak (quercetin), No.11 peak (resveratrol), No.12 peak (quercetin-3-rhamnoside), No.13 peak (emodin-8-O-β-D-glucoside), No.16 peak (emodin), No.18 peak (emodin methyl ether). Conclusion The effect of P. cuspidatum on gouty arthritis is the result of many components. This study can provide reference for the quality control and application development of this medicine.

R286.2

国家重点研发计划中医药现代化研究重点专项资助项目（2017YFC1701003）