目的 研究矢镞叶蟹甲草Parasenecio rubescens全草的化学成分。方法 采用硅胶柱色谱、MCI小孔树脂柱色谱、Sephadex LH-20凝胶柱色谱及半制备高效液相色谱法对矢镞叶蟹甲草全草化学成分进行分离纯化，并通过NMR等波谱学技术鉴定所分离得到的单体化合物的结构；运用MTT法测试细胞毒活性。结果 从矢镞叶蟹甲草90%丙酮提取物中分离得到10个化合物，分别鉴定为7R，8S-3-（3，4-methylenedioxybenzyl）-7，9-diangeloyloxy-5'-methoxy-9'-hydroxy-8-O-3'-neolignan（1）、黄体酮（2）、孕甾-1，4-二烯-3，20-二酮（3）、β-谷甾醇（4）、胡萝卜苷（5）、珊瑚菜内酯（6）、异茴芹素（7）、咖啡酸乙酯（8）、（+）-annuionone D（9）、（−）-asperentin（10）。结论 化合物1~10均为首次从该植物中分离得到。化合物1为1种新的8-O-3'型新木脂素，命名为矢镞叶蟹甲草素W。化合物2和3为植物来源的较少见的孕甾烷类成分。化合物1在给药浓度为100 μmol/L时，对小鼠黑色素瘤B16细胞、人肝癌HepG2细胞、人乳腺癌MCF7细胞均无明显细胞毒活性。

Objective To study the chemical constituents from the whole plant of Parasenecio rubescens. Methods The chemical constituents were isolated and purified by using silica gel, MCI, and Sephadex LH-20 column chromatography, as well as semi-preparative HPLC. Their structures were elucidated by analysis of spectroscopic and reported data. MTT method was used for cytotoxic activity analysis. Results A total of 10 compounds were isolated from the acetone extract of P. rubescens, and they were identified as 7R,8S-3-(3,4-methylenedioxybenzyl)-7,9-diangeloyloxy-5'-methoxy-9'-hydroxy-8-O-3'-neolignan (1), progesterone (2), pregna-1,4-diene-3,20-dione (3), β-sitosterol (4), daucosterol (5), phelloptorin (6), isopimpinellin (7), ethyl caffeate (8), (+)-annuionone D (9), and (−)-asperentin (10), respectively. Conclusion Compounds 1-10 are isolated from P. rubescens for the first time. Compound 1 is a new neolignan, named pararubin W, and compounds 2 and 3 are plant-derived steroidal pregnanes, which are scarce in plant resources. Compound 1 showed no cytotoxic activity against mouse melanoma B16 cells, human hepatoma HepG2 cells and human breast cancer MCF7 cells at 100 μmol/L.

R284.1

国家自然科学基金面上项目（22077041）；湖南省教育厅优秀青年项目（18B531）；湖南省自然科学基金青年项目（2019JJ50422）