目的 建立厚朴Magnolia officinalis、凹叶厚朴M. officinalis var. biloba 2种基原厚朴药材的超高效液相色谱（UPLC）指纹图谱，为厚朴药材的质量控制提供参考。方法 采用UPLC法，色谱柱为Waters Acquity UPLC^®BEH C₁₈（100 mm×2.1 mm，1.7 μm）；流动相为乙腈-0.4%磷酸水溶液，梯度洗脱；体积流量为0.4 mL/min；波长294 nm；柱温30℃；进样量为1 μL。通过相似度评价、聚类分析（cluster analysis，HCA）、主成分分析（principal component analysis，PCA）、正交偏最小二乘法-判别式分析（orthogonal partial least squares method-discriminant analysis，OPLS-DA）和t检验对2种基原厚朴的指纹图谱进行评价。结果 厚朴、凹叶厚朴指纹图谱均具有9个共有峰，通过与对照品比对，指认出其中2个共有峰，分别为厚朴酚、和厚朴酚。13批厚朴指纹图谱与对照指纹图谱的相似度为0.907～0.993，6批凹叶厚朴与厚朴对照指纹图谱的相似度为0.770～0.895；CA将19批厚朴归为3类，并结合PCA和OPLS-DA发现4个差异性标志物，经t检验，2种基原厚朴4种差异性标志物的含量具有显著差异（P＜0.05）；凹叶厚朴中峰2、4、7代表的化学成分含量高于厚朴，而厚朴中和厚朴酚含量要高于凹叶厚朴。结论 该方法操作简单，专属性强，为2种基原厚朴药材的区分和质量控制提供参考。

Objective To establish the UPLC fingerprints of Houpo (Magnoliae Officinalis Cortex) from two different origins (Magnolia officinalis and M. officinalis var. biloba) and provide reference for quality control of Magnoliae Officinalis Cortex. MethodsUPLC Method was adopted. The determination was performed on a column of Waters Acquity UPLC^®BEH C₁₈ (100 mm×2.1 mm，1.7μm) with acetonitrile-0.4% phosphoric acid solution as the mobile phase by gradient elution at a flow rate of 0.4 mL/min. The detective wavelength was 294 nm, the column temperature was 30 ℃, the injection volume was 1 μL. Similarity evaluation, cluster analysis (HCA), principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA) and t-test were used to evaluate the fingerprints of Magnoliae Officinalis Cortex from two different origins. ResultsThere were nine common peaks in the fingerprints of Magnoliae Officinalis Cortex from two different origins. Two of them were identified as honokiol and magnolol by comparing with the reference substance. The fingerprint similarity between 13 batches of Magnoliae Officinalis Cortex and the control was 0.907—0.993. The fingerprint similarity between six batches of Magnolia officinalis var. biloba and M. officinalis was 0.770—0.895. According to the analysis of CA,19 batches of Magnoliae Officinalis Cortex were divided into three categories. Four kinds of differential markers were found by PCA and OPLS-DA. By t-test, there were significant differences in the contents of four differential markers between the two different origins(P< 0.05). The content of chemical components represented by peaks 2, 4 and 7 in M. officinalis var. biloba was higher than that in M. officinalis. The content of honokiol in M. officinalis was higher than that in M. officinalis var. biloba. ConclusionThis method is simple and specific, which can provide reference for the differentiation and quality control of two kinds of Magnoliae Officinalis Cortex.

R286.2

广东省省级科技计划项目（2018B030323004）；“广东特支计划”科技创业领军人才项目（2017TY04R197）