[关键词]
[摘要]
目的 对米泔水制北苍术的最佳炮制工艺进行优化,并对其抗腹泻作用进行研究。方法 以单因素试验为基础,结合Box-Behnken设计-响应面法,以苍术素、浸出物和外观性状为综合评价指标,对米泔水用量、闷润时间、炒制温度和炒制时间4个因素进行考察,确定米泔水制北苍术的最佳炮制工艺。建立脾虚泄泻症大鼠模型,比较给药前后大鼠体质量和粪便含水率变化,并采用酶联免疫吸附法测定血清中二胺氧化酶(diamine oxidase,DAO)和分泌型免疫球蛋白(secreted immunoglobulin,SIgA)含量。结果 响应面法得出米泔水制北苍术的最佳炮制工艺为米泔水中米水比例为1∶60,米泔水用量25 mL,炒制温度160 ℃,炒制时间30 min;与模型组比较,高剂量组米泔水制北苍术醇提取物可使脾虚泄泻大鼠的粪便含水率和DAO含量显著降低(P<0.01),体质量和SIgA含量显著增加(P<0.01),且接近阳性药水平;生苍术醇提取物对脾虚泄泻大鼠的粪便含水率、体质量、SIgA和DAO含量改善效果低于米泔水制北苍术(P<0.05、0.01)。结论 响应面法简便稳定、准确可靠,可用于优化米泔水制北苍术炮制工艺,且米泔水制北苍术醇提物的抗腹泻作用药效显著。
[Key word]
[Abstract]
Objective To optimize the processing technology of Atractylodes chinensis (AC) with rice water, and to study its anti-diarrhea efficacy. Methods Based on single factor test, the Box-Behnken response surface methodology was also used to determine the optimal processing technology of AC preparation with rice water investigated four factors, including the amount of rice water, moistening time, processing temperature and processing time, of which atractydin, extract and appearance traits were used as comprehensive evaluation indexes. Furthermore, with the establishment of spleen deficiency diarrhea model in rats, the changes of body weight and fecal moisture content before and after administration were compared, and the contents of diamine oxidase (DAO) and secreted immunoglobulin (SIgA) in serum were determined by enzyme-linked immunosorbent assay. Results Response surface methodology showed that the best processing technology of AC with rice water was as follows: The ratio of rice to water in rice water was 1:60, the amount of rice water was 25 mL, the processing temperature was 160 ℃, and the processing time was 30 min. Compared with model group, the content of fecal moisture content and DAO were significantly decreased (P < 0.01), meanwhile the body weight and SIgA content were significantly increased (P < 0.01) in high dose ethanol extract group of AC with rice water, which were close to the positive drug level to rats with spleen deficiency diarrhea. The effect of ethanol extract in raw products of AC on improving fecal moisture content, body weight, SIgA and DAO content in rats with spleen deficiency was lower than that of rice water treatment (P < 0.05, 0.01). Conclusion Response surface method is simple, stable, accurate and reliable, which can be used to optimize the processing technology of AC prepared with rice water, and the anti-diarrhea effect of ethanol extract from AC with rice water is significant.
[中图分类号]
R283.1
[基金项目]
国家自然科学基金资助项目(81803649);吉林省科技厅科技项目(20191102035YY);吉林省中医药管理局科技项目(2020047)