目的 研究采用不同尺寸和材质针式微孔滤膜处理中药饮片，对其中指标性成分野黄芩苷、黄芩苷和甘草酸铵HPLC定量检测结果的影响，以确定最适用的微孔滤膜类型。方法 以中药饮片半枝莲、黄芩、甘草为研究对象，采用安谱、津腾、泰坦、岛津等不同品牌尼龙、聚四氟乙烯（PTFE）等不同材质及0.45、0.22μm等不同尺寸的有机相微孔滤膜滤过以及高速离心进行样品前处理，对3种中药成分（野黄芩苷、黄芩苷、甘草酸铵）分别按《中国药典》2020年版方法进行HPLC定量测定；同时还测定了经上述不同方法处理的不同质量浓度水平（低、中、高）对照品溶液，得到的相应色谱峰的响应值。采用主成分分析（principal component analysis，PCA）进行统计分析，比较采用不同微孔滤膜的处理方式对各定量测定结果的影响。结果 与经高速离心处理后的样品测定结果相比，不同样品前处理的对照品溶液峰面积均呈现不同程度的降低。 结论 微孔滤膜对3种指标成分均具有不同程度的吸附作用；采用PTFE滤膜处理后的含量结果、峰面积结果与高速离心法更接近，对定量测定结果影响更小，更适用于进样前样品（对照品、供试品）溶液的滤过处理。

Objective To study the influence of millipore filters of different sizes and materials on the determination results of scutellarin, baicalin and ammonium glycyrrhizinate detected by HPLC, and to determine the most appropriate millipore filters applied in the filtration before injected into HPLC analyzers. Methods Three kinds of traditional Chinese medicine (TCM) decoction pieces [Banzhilian (Scutellariae Barbatae Herba, SBH), Huangqin (Scutellariae Radix, SR), Gancao (Glycyrrhizae Radix et Rhizoma, GRR)] were used as the investigated subjects. The millipore filters with different brands (Anpel, Jinteng, Titan, Shimadzu), with different materials (Nylon, PTFE) and different micropore sizes (0.45, 0.22 μm) were used to filter the sample solutions, with the high-speed centrifugation as the reference treatment, the contents of the indicative components (scutellarin, baicalin, ammonium glycyrrhizinate) in the corresponding decoction pieces (SBH, SR, GRR) were detected according to the respective content-determination methods listed in Chinese Pharmacopoeia (2020 edition), respectively. Additionally, the response values of the corresponding peaks of the reference solutions with different concentration levels (low, medium, high) were obtained, which were treated with the above-mentioned different methods, respectively. Principal component analysis (PCA) was used for statistical analysis, and the influences of different millipore filters on the quantitative determination results were compared. Results Compared with the determination results of the samples treated by high-speed centrifugation, the peak areas of the reference solutions responded in HPLC, showed different degrees of reduction, which led to the simultaneous increase of the corresponding determination results of the decoction pieces. Conclusion All the above microporous filters have different degrees of adsorption on the three index components. The results of content and peak area after the treatment with PTFE filters (0.22 μm or 0.45 μm), are closer to those treated by high-speed centrifugation, which indicate less influence of the filters on the quantitative determination results. PTFE millipore filters are more suitable for pre-treatment of the reference/test sample solutions in the HPLC determinations of the contents of scutellarin, baicalin or ammonium glycyrrhizinate.

R283.6

“重大新药创制”科技重大专项（2019ZX09201004-002）；国家自然科学基金青年科学基金项目（82003962）；上海市科委“科技创新行动计划”生物医药领域科技支撑项目（1841931400）