[关键词]
[摘要]
目的 研究缬草Valeriana officinalis的化学成分及其细胞毒活性。方法 采用硅胶柱色谱、葡聚糖凝胶柱色谱以及半制备HPLC等方法进行分离纯化,根据理化性质、MS、NMR和ECD数据鉴定化合物的结构,采用MTT法测定化合物的细胞毒活性。结果 从缬草中分离得到15个化合物,分别鉴定为缬草木脂素A(1)、(7R,8S,8'R)-tetrahydro- 7-(4-hydroxy-3-methoxyphenyl)-8'-[(4'-hydroxy-3'-methoxyphenyl)methyl]-8-(hydroxylmethyl)-8,8'-furandiol(2)、schisanpropinin(3)、(+)-异落叶松脂素(4)、ecdysanol B(5)、(−;)-2-(3'-甲氧基-4'-羟基-苯基)-3,4-二羟基-4-(3″-甲氧基-4″-羟基-苄基)-3-四氢呋喃甲醇(6)、jatamanin A(7)、jatamanin K(8)、jatamanin R(9)、longiflorone(10)、阿魏酸(11)、咖啡酸(12)、对香豆酸(13)、咖啡酸甲酯(14)、对羟基肉桂酸甲酯(15),化合物10对人结肠癌HCT116细胞具有细胞毒活性,半数抑制浓度(median inhibition concentration,IC50)值为(70.9±6.4)μmol/L。结论 化合物1为新化合物,化合物2、3、5~10首次从缬草中分离得到,化合物10具有一定的细胞毒活性。
[Key word]
[Abstract]
Objective To study the chemical constituents from Valeriana officinalis and their cytotoxic activities. Methods The compounds were separated by silica gel column chromatography, Sephadex LH-20 gel column chromatography and semi-preparative HPLC. The structures were identified by their physicochemical properties, MS, 1D and 2D NMR spectra and ECD data analysis. The cytotoxic activities were tested by MTT assay. Results Fifteen compounds were isolated from V. officinalis, which were identified as: valeofficinlignan A (1), (7R,8S,8'R)-tetrahydro-7-(4-hydroxy-3-methoxyphenyl)-8'-[(4'-hydroxy-3'-methoxyphenyl)methyl]-8- (hydroxylmethyl)-8,8'-furandiol (2), schisanpropinin (3), (+)-isolariciresinol (4), ecdysanol B (5), (‒)-3,4-dihydroxy-2- (4-hydroxy-3-methoxyphenyl)-4-(4-hydroxy-3-methoxybenzyl)-3-tetrahydrofuranmethanol (6), jatamanin A (7), jatamanin K (8), jatamanin R (9), longiflorone (10), ferulic acid (11), caffeic acid (12), p-coumaric acid (13), methylcaffeate (14), and methyl p-hydroxycinnamate (15). Compound 10 showed cytotoxic activity against HCT116 cell line with IC50 value of (70.9 ±6.4) μmol/L. Conclusion Compound 1 is a new monoepoxylignan, namely valeofficinlignan A, and compounds 2, 3, 5―10 are isolated from V. officinalis for the first time, compound 10 exhibits cytotoxic activities.
[中图分类号]
R284.1
[基金项目]
陕西省科技厅自然科学基础研究计划项目(2021JQ-744);陕西中医药大学创新团队项目(2019-YL12)