目的 研究益母草Leonurus japonicus化学成分及其活性。方法 采用硅胶、ODS、凝胶等色谱填料，半制备高效液相色谱等方法进行分离纯化，根据化合物的理化性质、MS、1D-NMR和2D-NMR等波谱数据鉴定结构。采用脂多糖（lipopolysaccharide，LPS）诱导RAW264.7细胞产生一氧化氮（NO）的模型评价化合物活性。结果 从益母草中分离得到6个化合物，分别鉴定为 （−；）-3b，15-二羟基-16-丁香酰氧基-半日花烷-8（9），13（Z）-二烯-7-酮-3-O-b-D-葡萄糖苷（1）、环-（L-脯氨酸-L-脯氨酸）（2）、2，3-二氢-4（1H）-喹诺酮（3）、3，4，5-三甲氧基苯甲酰胺（4）、水苏碱（5）、蔗糖（6）。化合物1可抑制LPS诱导RAW264.7细胞产生NO。结论 化合物1为1个新化合物，命名为益丁香酰益母草二萜苷，其对LPS诱导RAW264.7细胞释放NO有抑制活性。化合物2～4、6为首次从益母草中分离得到。

Objective This paper aims to investigate chemical constituents and bioactivity from the ariel parts of Leonurus japonicus. Methods Chromatography using silica gel columns, ODS columns and semi-preparative high performance liquid chromatography were mainly applied to isolation and purification of compounds. The structures were elucidated by their physicochemical properties, MS, 1D-NMR and 2D-NMR spectral data. Bioactivity was evaluated by a model of LPS-induced NO production in RAW264.7 cells. Results Six compounds were isolated and identified as (−)-3β,15-dihydroxy-16-syringyloxy- labda-8(9),13(Z)-dien-7-one-3-O-β-D-glucoside (1), cyclo-(L-Pro-L-Pro) (2), 2,3-dihydro-4(1H)-quinolone (3), 3,4,5-trimethoxy- benzamide (4), stachydrine (5), and sucrose (6). Compound 1 inhibited LPS-induced NO production in RAW264.7 cells. Conclusion Compound 1 has a new structure, named syringylleojaposide, which showed inhibitory activity against NO production in LPS-induced RAW264.7 cells. The compounds 2—4, and 6 are isolated from L. japonicus for the first time.

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国家优秀青年基金资助项目（82022072）；国家自然科学基金面上项目（81872991）；国家自然科学基金联合基金重点支持项目（U19A2010）；国家自然科学基金青年基金资助项目（81303209）