目的 鉴定艾叶Artemisiae Argyi Folium中主要化学成分的结构并建立其含量测定方法。方法 利用UPLC-UV-Q/TOF进行艾叶分析条件的优化，在对21批艾叶分析的基础上，确定其中紫外和质谱上主要的色谱峰，利用精确相对分子质量和碎片离子鉴定其结构后，利用对照品比对确定结构；进一步采用Waters Acquity I-Class^TM液相色谱系统，Waters Acquity HSS T3色谱柱（100 mm×2.1 mm，1.8 μm），乙腈-0.2%磷酸水为流动相，体积流量0.5 mL/min，梯度洗脱，柱温40℃，PDA检测器，检测波长为326 nm，建立艾叶中主要化学成分的含量测定方法。结果 推测鉴定了艾叶中32个主要化合物的结构，利用对照品确定了其中15个化合物的结构，并建立了其中14个化合物（绿原酸、新绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、咖啡酸、夏佛塔苷、芹菜素、高车前素、棕矢车菊素、异泽兰黄素、5，7，3'-三羟基-6，4'，5'-三甲氧基黄酮、蔓荆子黄素）的UPLC-UV含量测定方法。14个化合物的分离度良好，稳定性和重复性良好，且线性范围宽（0.5～800.0μg/mL）、线性较好（R²>0.999），平均回收率在94.88%～103.58%。结论 建立的艾叶中14个主要化合物的含量测定方法可以全面评价艾叶的质量。

Objective To identify the structures of the main chemical constituents of Artemisiae argyi Folium and establish a method for their content determination. Methods UPLC-UV-Q/TOF was used to optimize the conditions for the analysis of Artemisia argyi. On the basis of the analysis of 21 batches of Artemisia argyi Folium, the main chromatographic peaks on ultraviolet and mass spectra were found. The structures were identified by accurate molecular weight and fragment ions. The structures were determined by comparison of standards. The structures were further determined by UPLC-UV and Waters Acquity I-Class^TM solution. A method for content determination was established by using Waters Acquity HSS T3 column (100 mm×2.1 mm, 1.8 um), acetonitrile-0.2% phosphoric acid water as mobile phase, flow rate gradient elution at 0.5 mL/min, the column temperature was 40℃, PDA detector, detection wavelength was 326 nm. Results The structures of the main compounds in 32 of Artemisia argyi Folium were identified, and 15 of them were identified by reference substances. The UPLC-UV method for simultaneous determination of 14 compounds (chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, isochlorogenic acids A-C, caffeic acid, chaffaloside, apigenin, plantain, cypermethrin, and isozelan yellow) in Artemisia argyi Folium were established. The 14 compounds have good separation, stability and repeatability, and wide linear range (0.5-800 μg/mL) and good linearity (R²>0.999). The average recovery was 94.88%-103.58%. Conclusion The established method for the determination of 14 main compounds in Artemisia argyi Folium can evaluate the quality of Artemisiae Argyi Folium.

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国家重点研发计划（2020YFC1712700）；中国中医科学院科技创新工程项目（CI2021A05206）；中央本级重大增减支项目—名贵中药资源可持续利用能力建设（2060302）