目的 建立野菊花Chrysanthemum indicum药材HPLC指纹图谱，并进行化学模式识别。方法 采用Hypersil ODS C₁₈（250 mm×4.6 mm，5 μm）色谱柱；流动相为乙腈-0.1%磷酸，梯度洗脱，检测波长334 nm；体积流量1 mL/min；柱温30℃；进样量10 μL，建立野菊花药材HPLC指纹图谱，结合相似度评价、聚类分析、主成分分析和正交偏最小二乘-判别分析等模式识别方法，对不同产地野菊花药材进行质量评价。结果 建立的指纹图谱方法符合方法学要求，29批野菊花药材HPLC指纹图谱有10个共有峰，相似度均在0.91以上；通过聚类分析将样品按不同产地分为2大类；主成分分析与聚类分析结果一致；最后采用正交偏最小二乘-判别分析筛选出了不同批次野菊花药材的6个差异标志物，通过对照品指认，确定了10号峰为蒙花苷、5号峰为3，4-二咖啡酰奎宁酸、3号峰为木犀草苷、1号峰为绿原酸。结论 建立的指纹图谱方法稳定、可靠、重复性好；结合化学模式识别可用于野菊花药材的质量品质评价。

Objective To establish an HPLC fingerprint of Chrysanthemum indicum medicinal materials and perform chemical pattern recognition. Methods Hypersil ODS C₁₈ (250 mm×4.6 mm, 5 μm) chromatographic column was used; Mobile phase was acetonitrile-0.1% phosphoric acid, gradient elution; Detection wavelength was 334 nm; Flow rate was 1 mL/min; Column temperature was 30℃; Injection volume was 10 μL. HPLC fingerprints of C. indicum medicinal materials was established, combined with similarity evaluation, cluster analysis, principal component analysis and orthogonal partial least squares-discriminant analysis and other pattern recognition methods, quality evaluation of C. indicum medicinal materials from different places. Results The established fingerprint method met the method requirements. The 29 batches of C. indicum medicinal materials had 10 common peaks in the HPLC fingerprint, and the similarity was all above 0.91; The samples were divided into two categories according to different origins through cluster analysis; The results of principal component analysis and cluster analysis were consistent; Finally, the orthogonal partial least squares-discriminant analysis was used to screen out six different markers of different batches of wild chrysanthemum medicinal materials. Through the identification of the reference substance, it was determined that peak 10 was montmorrin and peak 5 was 3,4-dicaffeoylquinic acid, peak 3 was luteolin, peak 1 was chlorogenic acid. Conclusion The established fingerprint method is stable, reliable, and reproducible; Combined with chemical pattern recognition, it can be used for the quality evaluation of C. indicum medicinal materials.

R286.2

江西中医药大学中药学一流学科专项科研基金项目（5251800280）；2019年度江西省中医药管理局科技计划项目（2019A005）