目的 测定不同产地槐角Sophorae Fructus中槐角苷、芦丁、染料木苷含量，并建立其HPLC指纹图谱，结合化学模式识别建立槐角有效的质量评价方法，为槐角药材的质量标准制定提供依据。方法 采用Agilent Zorbax SB C₁₈色谱柱在检测波长为260 nm，体积流量为0.6 mL/min，甲醇-乙腈-0.07%磷酸水（12∶20∶68）为流动相进行等度洗脱，并运用中药色谱指纹图谱相似度评价系统结合化学模式识别技术进行数据分析，确定共有峰并筛选差异性成分。结果 10个不同产地槐角样品的指纹图谱中有9个共有峰为特征峰，与对照图谱相似度均在0.98以上。不同产地槐角的槐角苷、芦丁、染料木苷平均含量分别为8.9%、4.2%、6.45%。聚类分析将10批不同产地的槐角分为2类，主成分分析、正交偏最小二乘-判别分析结果与聚类分析一致，并筛选出不同批次的差异性成分为9、1、2、6、3号峰，其中9、6号峰分别指认为槐角苷、染料木苷。结论 建立了槐角的HPLC指纹图谱及化学模式识别下寻找不同产地槐角质量差异成分的方法，为全面评价槐角药材的质量提供有效手段。

Objective To determine the content of Sophorae Fructus, rutin and genistein in Sophorae Fructus from different cultivation places, and establish their HPLC fingerprint. Combined with chemical pattern recognition, an effective quality evaluation method of Sophorae Fructus was established to provide basis for the quality research of Sophorae Fructus. Methods The ethanol extract of Sophorae Fructus was eluted on Agilent Zorbax sb C₁₈ column at the detection wavelength of 260 nm and the flow rate of 0.6 mL/min. The mobile phase consisted of methanol-acetonitrile-0.07% phosphoric acid water (12:20:68). The similarity evaluation system of chromatographic fingerprint of traditional Chinese medicine and chemical pattern recognition technology were used for data analysis to determine the common peaks and screen the different components. Results Nine common peaks were characteristic peaks in the fingerprints of 10 Sophorae Fructus samples from different cultivation places, and the similarity with the control fingerprint was more than 0.98. The average contents of Sophoricoside, rutin and genistein in Sophorae Fructus from different habitats were 0.89%, 0.42% and 6.45%, respectively. The results of principal component analysis and orthogonal partial least squares discriminant analysis were consistent with those of cluster analysis. The difference components of different batches were selected as 9, 1, 2, 6 and 3, among which peaks 9 and 6 refer to Sophoricoside and genistein respectively. Conclusion In this paper, HPLC fingerprint and chemical pattern recognition method were established to find the quality difference components of Sophorae Fructus from different habitats, which provided an effective means for comprehensive evaluation of the quality of Sophorae Fructus.

R286.2

吉林省地方药材标准化项目-炒槐角（PZ-2016-71）