目的 分析当归Angelica sinensis中阿魏酸和藁本内酯等主要有效成分在其根部的积累规律和转化机制。方法 采用HPLC法测定不同生长期当归根部中阿魏酸、阿魏酸松柏酯和藁本内酯等8种苯酞类挥发油成分的含量，结合体外转化研究成果进行分析。结果 阿魏酸和阿魏酸松柏酯含量从苗期到根膨大期沿着相同的方向不断增加，而从根膨大期到收获期分别逐渐降低和升高，朝2个相反的方向变化，提示在当归体内可能存在阿魏酸和松柏醇转化为阿魏酸松柏酯的过程。藁本内酯和5种转化产物含量总体随生长期呈现上升趋势，尤其从根膨大期到成药期增长速度极快，符合成药期特性；藁本内酯含量远远大于其他6种苯酞类转化产物洋川芎内酯I、洋川芎内酯H、阿魏酸松柏酯、洋川芎内酯A、正丁基苯酞、欧当归内酯的含量，提示在当归活体内藁本内酯的转化程度不高，具体转化机制有待进一步研究。结论 阿魏酸和藁本内酯在不同生长期具有不同的积累和转化规律，可为当归药材生产、炮制和质量评价提供理论支持。

Objective To analyze the accumulation rule and possible transformation mechanism of ferulic acid and ligustilide, the main pharmacodynamic components of Angelica sinensis root. Methods The contents of ferulic acid, coniferyl ferulate and six phthalides in roots of A. sinensis at different growth stages were analyzed by HPLC combined with the results of in vitro transformation studies. Results The content of ferulic acid and coniferyl ferulate increased in the same direction from seedling stage to root enlargement stage, while gradually decreased and increased from root enlargement stage to harvest stage changing in two opposite directions. It was suggesting that there may be a process of conversion of ferulic acid and coniferyl alcohol into coniferyl ferulate in the root of A. sinensis. The content of ligustilide and five transformed products showed an increasing trend with the growth period, especially from the root enlargement stage to the medication stage, which accorded with the characteristics of medication stage. The content of ligustilide was much greater than that of the other five phthalides, suggesting that the transformation degree of ligustilide in A.sinensis in vivo was not high, and the specific transformation mechanism needs to be further clarified. Conclusion Ferulic acid and ligustilide have different accumulation and transformation rules in different growth periods. This study can provide theoretical support for the production, processing and quality evaluation of A. sinensis medicinal materials.

R286.2

国家重点研发计划项目“中医药现代化研究”专项（子课题）（2017YFC1700705）；甘肃省教育厅双一流重大科研项目（GSSYLXM-05）；甘肃中医药大学扶贫科技专项（2019FPZX-1）；甘肃省教育厅高等学校创新能力提升项目（2019B-101）；甘肃中医药大学中青年基金项目（ZQ2015-5）