目的 研究多花黄精多糖对斑马鱼幼鱼2型糖尿病合并骨质疏松症的作用。方法 用链脲佐菌素和泼尼松龙建立斑马鱼幼鱼2型糖尿病合并骨质疏松症模型，设置对照组、模型组、二甲双胍＋依替膦酸二钠组和多花黄精多糖（60、120、240 μg/mL）组，考察药物对斑马鱼早期发育毒性的影响；采用试剂盒测定各组幼鱼组织的糖含量；采用茜素红染色法考察各组斑马鱼头骨矿化面积和骨密度；采用qRT-PCR法检测各组斑马鱼骨质疏松相关基因Runx家族转录因子2b（Runx family transcription factor 2b，Runx2b）、Sp7转录因子（Sp7 transcription factor，Sp7）、I型胶原蛋白α2（collagen type I α2，col1a2）、富含半胱氨酸的酸性分泌蛋白质因子（cysteine-rich acidic secreted protein，sparc）和维生素D受体b（vitamin D receptor b，vdrb）mRNA表达情况。结果 显微注射链脲佐菌素和浸泡泼尼松龙能够提高幼鱼组织糖浓度，降低头骨矿化面积和骨密度。与模型组比较，多花黄精多糖显著上调斑马鱼幼鱼2型糖尿病合并骨质疏松症模型Runx2b、Sp7、vdrb、sparc、col1a2 mRNA表达水平（P<0.05、0.01、0.001）。结论 多花黄精多糖对斑马鱼幼鱼2型糖尿病合并骨质疏松症模型有较好的降血糖及抗骨质疏松症作用。

Objective To study the effect of polysaccharide from Polygonatum cyrtonema (PSP) on type 2 diabetes and osteoporosis in juvenile zebrafish. Methods A zebrafish juvenile type 2 diabetes model with osteoporosis was established with streptozotocin and prednisolone, which were divided into control group, model group, metformin + etidronate disodium group and PSP (60, 120, 240 μg/mL) groups, the effect of drugs on early developmental toxicity of zebrafish was investigated; Kit was used to determine the sugar content of juvenile fish tissues in each group; Alizarin red staining method was used to investigate the mineralized area and bone mineral density in bones of zebrafish in each group; qRT-PCR was used to detect osteoporosis-related genes Runx family transcription factor 2b (Runx2b), Sp7 transcription factor (Sp7), type I collagen α2 (col1a2), cysteine-rich acidic secreted protein (sparc) and vitamin D receptor b (vdrb) mRNA expressions in zebrafish. Results Microinjection of streptozotocin and immersion of prednisolone increased the sugar concentration of juvenile fish tissues, reduced the mineralized area and bone density of skull. Compared with model group, PSP significantly up-regulated mRNA expression levels of Runx2b, Sp7, vdrb, sparc and col1a2 in zebrafish juvenile type 2 diabetes mellitus with osteoporosis model (P < 0.05, 0.01, 0.001). Conclusion PSP has a good hypoglycemic and anti-osteoporosis effect on zebrafish juvenile with type 2 diabetes and osteoporosis.

R285.5

安徽省博士后研究人员科研活动经费资助项目（2020A396）；安徽省2020年度中央引导地方科技发展专项项目（202007d06020007）