[关键词]
[摘要]
目的 采用UPLC-Q-TOF-MS/MS技术对六味地黄苷糖片中的化学成分进行分析和鉴定。方法 色谱柱为Phenomenex Luna C18(250 mm×4.6 mm,5 μm),以乙腈-0.3%甲酸水溶液作为流动相,梯度洗脱,体积流量1 mL/min,柱温35℃,进样量10 μL;采用电喷雾离子源,负离子模式下采集数据,根据精确质荷比、二级碎片离子信息,结合对照品和文献数据鉴定六味地黄苷糖片中的化学成分。结果 共鉴定和推测出71个化合物,其中25个来自酒萸肉、19个来自熟地黄、31个来自牡丹皮、2个来自泽泻、3个来自茯苓、2个来自山药。其中22个化合物经对照品验证。结论 基本明确了六味地黄苷糖片的化学物质基础,为六味地黄苷糖片的质量控制及药效物质基础研究提供了参考。
[Key word]
[Abstract]
Objective To study the chemical constituents in Liuwei Dihuang gantang tablets (六味地黄糖苷片) by using UPLC-Q-TOF-MS/MS. Methods The chromatographic conditions were as follows:Phenomenex Luna C18 (250 mm×4.6 mm, 5 μm) column with acetonitrile and 0.3% formic acid as mobile phase, gradient elution, flow rate of 1 mL/min, The column temperature was 35 degrees, the injection volume was 10 L; mass spectrometry conditions:The data were collected by electrospray ion source (ESI) under negative ion mode. These components were further analyzed based on the accurate mass ratio, the two level fragment ion information, the reference materials and literature data. Results A total of 71 compounds were identified and speculated, 25 of which came from liquor cornus, 19 from Rehmannia Radix Praeparata, 31 from Mutan Cortex, 2 from Alismatis Rhizoma, 3 from Poria and 2 from Dioscoreae Rhizoma. Among them, 22 compounds were verified by reference substance. Conclusion The results basically clarified the chemical basis of Liuwei Dihuang gantang tablets, and provided a reference for the quality control and pharmacodynamic material basis of Liuwei Dihuang gantang tablets.
[中图分类号]
R284.1
[基金项目]
重大新药创制科技重大专项(2013ZX09402203)