目的 研究买麻藤Gnetum montanum藤茎的化学成分。方法 利用正相硅胶、Sephadex LH-20凝胶、中压制备液相色谱和半制备HPLC等多种柱色谱技术进行分离纯化，并综合运用ESI-MS、HR-ESI-MS、¹H-NMR、¹³C-NMR、DEPT、¹H-¹H COSY、HSQC、HMBC等方法鉴定化合物的结构。结果 从买麻藤藤茎的95%乙醇提取物中分离得到14个化合物，分别鉴定为（Z）-5-（2，4-二羟基-6-（4-羟基-3-甲氧基苯乙烯基）苯基）吡咯烷-2-酮（1）、银松素单甲醚（2）、3-羟基-5，4'-二甲氧基芪（3）、银松素（4）、买麻藤醇（5）、刚果买麻藤素F（6）、异丹叶大黄素（7）、闭苞买麻藤素（8）、白藜芦醇（9）、顺式-ε-葡萄素（10）、ε-葡萄素（11）、双异食用大黄苷元A（12）、顺式-射干素B（13）和小叶买麻藤素A（14）。结论 化合物1为新化合物，命名为顺式买麻藤内酰胺（cis-gnetumamide），化合物3、10、13和14为首次从该植物中分离得到。

Objective To study the chemical constituents from the stems of Gnetum montanum. Methods The compounds were isolated and purified by silica gel, Sephadex LH-20 gel column chromatography, preparative MPLC, and semi-preparative HPLC. Their structures were fully determined based on spectroscopic analysis including ESI-MS, HR-ESI-MS, ¹H-NMR, ¹³C-NMR, DEPT, ¹H-¹H COSY, HSQC and HMBC spectra. Results Fourteen compounds were isolated and identified from 95% ethanol extract of the stems of G. montanum. The structure of 1 was established as (Z)-5-(2,4-dihydroxy-6-(4-hydroxy-3-methoxystyryl) phenyl) pyrrolidin-2-one (1), and the other compounds were identified as pinosylvin monomethyl ether (2), 3-hydroxy-5,4'-dimethoxystilbene (3), pinosylvin (4), gnetol (5), gneafricanin F (6), isorhapontigenin (7), gnetulin (8), resveratrol (9), cis-ε-viniferin (10), ε-viniferin (11), bisisorhapontigenin A (12), cis-shegansu B (13) and gnetifolin A (14). Conclusion Compound 1 is a new compound and named cis-gnetumamide. Compounds 3, 10, 13 and 14 were isolated from the plant for the first time.

R284.1

国家发改委“大规模综合性化合物库建设”专项基金