目的 研究越南安息香（白花树Styrax tonkinensis的干燥树脂）的化学成分及其细胞毒活性。方法 采用MCI gel CHP-20P、硅胶、Sephadex LH-20柱色谱、快速制备色谱和制备HPLC等技术进行分离和纯化，通过IR、1D-和2D-NMR、HR-ESI-MS等谱学数据分析确定化合物的结构。通过CCK-8法评价了化合物的细胞毒性。结果 从越南安息香提取物中分离得到6个化合物，分别鉴定为（E）-3-[3-甲氧基-4-（3-乙氧基-2-氧代乙氧基）苯基]烯丙基苯甲酸酯（1）、2-（4-羟基-3-甲氧基苯基）-5-（3-羟丙基）-7-甲氧基苯并呋喃（2）、（＋）-雪松素（3）、（＋）-松脂素（4）、松脂素单甲醚（5）和安息香脂素（6）。以上6个化合物对人宫颈癌HeLa细胞株、人肝癌HepG-2细胞株、人乳腺癌MCF细胞株、人前列腺癌PC-3细胞株以及人肺癌A549细胞株的半数抑制浓度值均大于100 μmol/L。结论 化合物1为未见报道的新苯丙素类化合物，命名为白花树酯醚，化合物2～6为首次从越南安息香中分离得到。化合物1～6均未表现出明显的细胞毒活性。

Objective To study chemical constituents from the resin of Styrax tonkinensis (Pierre) Craib ex Hartw and their cytotoxicities. Methods The compounds were isolated by column chromatography over MCI gel CHP-20P, silica gel, Sephadex HL-20, and flash chromatography coupled with preparative HPLC. Their structures were elucidated by spectroscopic analysis including IR, 1D-NMR, 2D-NMR, and HR-ESI-MS. Furthermore, the cytotoxicities of the chemical components against HepG-2, A549, HeLa, MCF-7, and PC-3 cell lines were measured by CCK-8 method. Results Six compounds, (E)-3-[3-methoxy-4-(2-ethoxy-2-oxoethoxy)-phenyl]allyl benzoate (1), 2-(4-hydroxy-3-methoxyphenyl)-5-(3-hydroxypropyl)-7-methoxybenzofuran (2), (+)-cedrusin (3), (+)-pinoresinol (4), (+)-pinoresinol monomethy ether (5), and styraxin (6) were isolated from the resin of S. tonkinensis. And the IC₅₀ values of the six compounds to HeLa, HepG-2, MCF, PC-3 and A549 cell lines were more than 100 μmol/L. Conclusion Compound 1 is identified as a new phenylpropanoid named as stytonkinether, and compounds 2-6 are isolated from the resin of this plant for the first time. And all six compounds show no obviously cytotoxic activities.

R284.1

广东省自然科学基金面上项目（2021A1515011529）