目的 建立基于谱效关联分析的竹叶椒挥发油抗菌质量标志物（quality marker，Q-Marker）的研究方法，为其质量控制提供参考依据。方法 采用气相色谱-质谱联用法（GC-MS）建立竹叶椒挥发油指纹图谱，并进行不同产地竹叶椒相似度评价、聚类分析和主成分分析；考察不同产地竹叶椒挥发油对大肠杆菌、金黄色葡萄球菌、红色毛癣菌及须鲜毛癣菌的作用；采用灰色关联分析法探究竹叶椒挥发油中的化学成分与抗菌活性的相关性，筛选Q-Marker。结果 10批不同产地竹叶椒挥发油相似度良好，相似度均大于0.97。不同产地竹叶椒挥发油对金黄色葡萄球菌、大肠杆菌、红色毛癣菌和须鲜毛癣菌均具有抑制作用。灰色关联分析表明，柠檬烯与金黄色葡萄球菌关联度值较高，芳樟醇、侧伯酮与大肠杆菌、红色毛癣菌和须鲜毛癣菌关联度值较高。结论 柠檬烯、芳樟醇和侧柏醇可作为竹叶椒挥发油抗菌的潜在Q-Marker。

Objective To established a research method of antibacterial quality marker (Q-Marker) for volatile oil of Zanthoxylum armatum (VOZA) based on spectral effect correlation analysis, and provide a reference for its quality control. Methods Gas chromatography mass spectrometer (GC-MS) was used to establish fingerprint of VOZA, similarity evaluation, cluster analysis and principal component analysis of VOZA from different areas were carried out. Effect of different batches of VOZA on Escherichia coli, Staphylococcus aureus, Trichophyton rubrum and Trichophyton mentagrophytes were detected. Grey correlation analysis method was used to explore the correlation between chemical components and antibacterial activity to screen Q-Marker. Results Similarity of 10 batches of VOZA from different areas were all more than 0.97. VOZA had a significant inhibitory effect on S. aureus, E. coli, T. rubrum and T. mentagrophytes. Grey correlation analysis method showed that limonene had the strongest correlation with S. aureus; linalool and thujone had a strong association with E. coli, T. rubrum and T. mentagrophytes. Conclusion Limonene, linalool and thujone could be used as potential antibacterial Q-Marker of VOZA.

R285.5

国家自然科学基金面上项目（81573563）；国家自然科学基金面上项目（81674168）；四川省重点研发计划项目（20ZDYF3291）；四川省科技项目（2019YFS0157）；教育部重点实验室开放课题（KF2020009）；四川省中医药管理局花椒专项项目（2018HJZX014）；西南民族大学研究生创新型科研项目（CX2019SZ166）