目的 研究洋金花（白花曼陀罗Datura metel干燥花）95%乙醇提取物的生物碱成分。方法 采用大孔吸附树脂、硅胶、ODS、Sephadex LH-20等柱色谱和反相高效液相色谱方法对洋金花化学成分进行系统分离和纯化，并通过HR-ESI-MS、NMR等波谱学技术鉴定化合物的结构。结果 从洋金花乙醇提取物中分离得到13个生物碱类化合物，分别鉴定为3-hydroxy-1-（4-hydroxyphenethyl） pyrrolidine-2，5-dione（1）、L-焦谷氨酸甲酯（2）、喹啉（3）、烟酸（4）、1H-咪唑-4-羧酸丁酯（5）、苯基-β-萘胺（6）、4-（羟甲基）-3-（4-羟基苯乙基）吡啶-2（1H）-酮（7）、半枝莲碱（8）、金色酰胺醇酯（9）、对羟基苯乙胺（10）、苯乙胺（11）、苯丙氨醇（12）、尿苷（13）。结论 化合物1～9、11 和12 为首次从曼陀罗属植物中分离得到，化合物10 和13 为首次从该植物中分离得到。

Objective To study alkaloid from the 95% ethanol extract of the dry flowers from Datura metel. Methods The compounds were systematically isolated and purified by using various chromatographic techniques including macroporous resins, silica gel, ODS and Sephadex LH-20 column chromatographies, as well as reversed phase high-performance liquid chromatography. Their chemical structures were elucidated based on HR-ESI-MS and NMR spectroscopic. Results Thirteen alkaloids compounds were obtained from the dry flowers of D. metel and identified as 3-hydroxy-1-(4-hydroxyphenethyl) pyrrolidine-2,5-dione (1), L-pyroglutamic acid methyl ester (2), quinoline (3), nicotinic acid (4), butyl 1H-imidazole-4-carboxylate (5), phenyl-β-naphthylamine (6), aristopyridinone A (7), scutebarbatine A (8), aurantiamide acetate (9), tyramine (10), phenethylamine (11), phenyl-alalninol (12) and uridine (13). Conclusion Compounds 1-9, 11 and 12 are found in the genus for the first time. Compound 10 and 13 are isolated from D metel for the first time.

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北京市科委G20工程创新研究（Z181100002218029）；国家自然科学基金资助项目（81872761）；天然药物活性物质与功能国家重点实验室开放课题（GTZK202001）