目的 建立一测多评法同时测定斑花黄堇Corydalis conspersa中盐酸小檗碱、比枯枯灵、原阿片碱、黄连碱4种生物碱成分的含量。方法 采用酸提碱沉法，二氯甲烷萃取斑花黄堇中的生物碱成分，以盐酸小檗碱、比枯枯灵、原阿片碱、黄连碱4种生物碱为指标成分，结合一测多评法学建立各成分与盐酸小檗碱的相对校正因子，计算各成分的相对保留时间，实现一测多评；同时测定4种生物碱成分的量。色谱柱为B18120405.25摇光Waters C₁₈（250 mm×4.6 mm，5 μm）；流动相为乙腈（A）-0.2%的醋酸水溶液（B），梯度洗脱，0～5 min，25% A；6～10 min，25%～30% A；11～15 min，30%～35% A；16～25 min，35%～25% A。检测波长为245 nm，体积流量1.0 mL/min，柱温30℃，理论塔板数以盐酸小檗碱计算不低于4000。结果 斑花黄堇药材各组织中盐酸小檗碱、原阿片碱、黄连碱的含量较高，不同采集地区根部4种生物碱的含量比茎、叶、花中高。结论 建立的一测多评法含量测定的方法方便可行，可作为斑花黄堇药材中盐酸小檗碱、黄连碱、原阿片碱、比枯枯灵质量检测的方法。

Objective To establish a QAMS method for the simultaneous determination of four alkaloids in Corydalis conspersa. Method The alkaloids from C. conspersa were collected by acid and alkali precipitation extracted through dichloromethane. Berberine hydrochloride, bicuculline, protopine, coptisine as reference substances combined with HPLC method were used to calculate the relative correction factors and relative retention time of each component compared to berberine hydrochloride, achieving the review of QAMS; Regression equation method was used to determine the quantity of four alkaloids. B18120405.25C₁₈ (250 mm×4.6 mm, 5 μm); acetonitrile-0.2% acetic acid aqueous solution, gradient elution (pH 4.8), detection wavelength 245nm, flow rate:1.0 mL/min, column temperature:30℃, calculating theoretical plate number of berberine hydrochloride should be more than 4000. Results The contents of berberine hydrochloride, protopine and coptisine in different tissues of C. conspersa were higher. The contents of four alkaloids in roots were higher than those in stems, leaves and flowers. Conclusions The HPLC-QAMS method is convenient and feasible, so it can be used for the quality control of berberine hydrochloride, coptisine, protopine and bicuculline in C. conspersa.

R282.6

青海省科技厅项目（2016-ZJ-903）；青海省青藏高原药用动植物资源重点实验室（2020-ZJ-Y40）；青海省青藏高原植物化学重点实验室（2020-ZJ-Y20）；民族药资源化学国家民族事务委员会-教育部重点实验室（云南民族大学，MZY2101）