目的 构建川芎Ligusticum chuanxiong超高效合相色谱（UPC²）特征图谱，与HPLC特征图谱进行比较，为川芎质量评价提供新的参考。方法 采用UPC²-PDA法，以CO₂为流动相，异丙醇-甲醇（3∶7）为助溶剂，梯度洗脱，建立川芎药材化学成分特征图谱；利用主成分分析和聚类分析方法，分析川芎药材品质与产地的关联性。结果 建立了不同产区川芎药材特征图谱，以藁本内酯为参照峰，确定了8个共有特征峰，同时指认了5种特征性成分；经主成分分析4个主成分因子的累积方差贡献率为93.097%，聚类分析将20批不同产地药材分为3大类。结论 建立的川芎药材UPC²特征图谱的不仅精密度、重复性、稳定性良好，而且在分析方法上优于HPLC特征图谱，还可以对不同产地川芎药材品质进行快速、有效地评估。

Objective To establish the specific chromatogram of Ligusticum chuanxiong hort. by ultra performance convergence chromatograpy (UPC²), and compared with that of HPLC, providing reference for the quality evaluation of Ligusticum chuanxiong hort.. Methods Ligusticum chuanxiong hort. separated on UPC²-PDA, with CO₂ as the mobile phase and isopropanol-methanol (3:7) as cosolvent by gradient elution, and established the specific chromatogram of chemical components of Ligusticum chuanxiong hort. Then The principal component analysis and cluster analysis were performed to explore the correlation between the origins and quality of Ligusticum chuanxiong hort.. Results Using ligustilide as control, 8 common specific chromatogram peaks were confirmed in the specific chromatogram of chemical constituents of Ligusticum chuanxiong hort. from different regions, and 5 characteristic ingredients were identified. The cumulative variance contribution rate of the 4 principal components was 93.097 through principal component analysis, Ligusticum chuanxiong hort. from different origins were classified into 3 classes by cluster analysis. and standard control. Conclusion The specific chromatogram of chemical components of Ligusticum chuanxiong hort. established in this study has good precision, repeatability and stability. In addition, the analytical method is superior to HPLC specific chromatogram, which can quickly and effectively evaluate the quality of Ligusticum chuanxiong hort. from different origins.

R286.2

国家重点研发计划资助（2017YED0501500）；现代农业产业技术体系建设专项资金资助（CARS-21）