目的 研究黄芪建中汤在正常大鼠胆汁中的代谢产物，并归纳主要代谢途径。方法 采用高效液相色谱-静电场轨道阱高分辨质谱（UHPLC-Q-Exactive MS）方法，结合Compound Discover 3.0软件等技术，系统分析黄芪建中汤在大鼠胆汁中的原型成分和代谢产物。结果 在大鼠体内共检出6个原型成分和88个代谢产物，主要代谢途径为氧化、还原、脱水、乙酰化、甲基化、硫酸化、葡萄糖醛酸化、葡萄糖苷结合等。结论 建立的高分辨质谱方法可以分析黄芪建中汤在大鼠胆汁中的代谢产物，初步阐明了黄芪建中汤的体内代谢特征，为其在体内发挥疗效的药效物质基础研究提供了依据。

To study the metabolites of Huangqi Jianzhong Decoction (黄芪建中汤) in the bile of normal rats by UHPLC-Q-Exactive MS, and summarize their main metabolic pathways. Methods In this study, UHPLC-Q-Exactive MS and Compound Discover 3.0 software was used to identify the prototype and metabolites of Huangqi Jianzhong Decoction in bile samples, and summarized their main metabolic pathways. Results A total of six prototypes and 88 metabolites were detected in bile. The main metabolic pathways were oxidation, reduction, dehydration, acetylation, methylation, sulfation, glucuronization, glucoside binding, etc. Conclusion The studies have established a high-resolution mass spectrometry method to analyze the metabolites of Huangqi Jianzhong Decoction in bile, preliminarily clarify the metabolism characteristics of Huangqi Jianzhong Decoction, and provide the basis for the study of the pharmacodynamic substance in vivo.

R284.1

国家自然科学基金资助项目（81703697）；山西中医药大学科技创新能力培育计划项目（2019PY-136）