目的 观察健骨丸对去势大鼠骨质疏松性骨折愈合的影响。方法 建立去势大鼠右胫骨骨折模型，随机分为对照组、模型组、仙灵骨葆胶囊（300 mg/kg）组和健骨丸低、中、高剂量（150、300、450 mg/kg）组，给药15、30 d后，检测各组大鼠血清中雌二醇（estradiol，E₂）、降钙素（calcitonin，CT）、骨钙素（bone gla protein，BGP）、血钙、血磷水平和碱性磷酸酶（alkaline phosphatase，AKP）活性；采用X-射线扫描各组大鼠右胫骨骨折处并测定骨密度；采用苏木素-伊红（HE）染色检测各组大鼠右胫骨骨折处病理变化。结果 健骨丸可以提高去势大鼠血清中E₂、CT、BGP、血钙、血磷水平和AKP活性；健骨丸可以促进断端骨痂生成，提高骨密度，促进骨折断端愈合。结论 健骨丸能够上调骨细胞活性，促进去势后大鼠骨折愈合以及骨形成。

Objective To observe the effect of Jiangu Pill (健骨丸) on osteoporotic fracture healing in ovariectomized rats. Methods The right tibia fracture model of ovariectomized rats was established and randomly divided into control group, model group, Xianling Gubao Capsule (300 mg/kg) group, low-, medium- and high-dose Jiangu Pill (150, 300, 450 mg/kg) groups. Fifteen and 30 d after administration, estradiol (E₂), calcitonin (CT), bone gla protein (BGP), blood calcium, blood phosphorus levels and alkaline phosphatase (AKP) activity in serum of rats in each group were detected; X-ray was used to scan the fractures of right tibia of rats in each group and bone mineral density was measured; Hematoxylin-eosin (HE) staining was used to detect the pathological changes in right tibial fractures. Results Jiangu Pill increased E₂, CT, BGP, blood calcium, blood phosphorus levels and AKP activity in serum of ovariectomized rats; Jiangu Pill promoted the formation of broken end callus, increased bone density, and promoted the healing of fractured ends. Conclusion Jiangu pill can up-regulate the activity of bone cells, promote fracture healing and bone formation in ovariectomized rats.

R285.5

江苏省中医院制剂工艺改进和质量标准提升项目（制剂部发展基金）（Y2020CX75）