[关键词]
[摘要]
目的 对蓝桉Eucalyptus globulus果实的化学成分进行研究。方法 采用硅胶柱色谱、SephadexLH-20凝胶柱色谱及半制备高效液相色谱法分离纯化,通过有机波谱方法鉴定化合物结构,采用MTT法测定部分化合物对人乳腺癌细胞MCF-7的细胞毒活性。结果 从蓝桉果实石油醚萃取物中分离得到15个化合物,分别鉴定为大黄酚(1)、eucarobustol E(2)、eucarobustol G(3)、eucalyptal A(4)、eucalyptal C(5)、eucalyptin C(6)、eucalyptin D(7)、eucalyptin E(8)、eucalrobusone A(9)、eucalrobusone C(10)、eucalrobusone F(11)、eucalrobusone U(12)、eucalyptone(13)、euglobal-Ia1(14)、euglobal-Ia2(15)。细胞毒活性结果显示,化合物6、7、10、12、13对MCF-7细胞具有明显细胞毒活性,20 μmol/L浓度下抑制率分别为71.83%、71.11%、70.00%、80.53%、79.87%,化合物12对MCF-7细胞显示出较强的细胞毒活性,其半数抑制浓度(IC50)为13.84 μmol/L。结论 分离得到的成分均为酚性成分,其中化合物1为首次从桉属植物中分离得到,化合物2、3为首次从该植物中分离得到,化合物9~15为首次从蓝桉果实中分离得到;大部分化合物在20 μmol/L的浓度下表现出较好的抗MCF-7细胞活性。
[Key word]
[Abstract]
Objective To isolate and identify the chemical constituents from the fruits of Eucalyptus globulus. Methods The monomer compounds were separated and purified by silica gel column chromatography, Sephadex LH-20 gel column chromatography and semi-preparative high performance liquid chromatography, and the structure of the compounds was identified by organic spectroscopy. MTT method was used to detect the cytotoxic activity of partial compounds on MCF-7 cells. Results Fifteen compounds were separated from the petroleum ether layer extract of E. globulus, which were identified as chrysophanol (1), eucarobustol E (2), eucarobustol G (3), eucalyptal A (4), eucalyptal C (5), eucalyptin C (6), eucalyptin D (7), eucalyptin E (8), eucalrobusone A (9), eucalrobusone C (10), eucalrobusone F (11), eucalrobusone U (12), eucalyptone (13), euglobal-Ia1 (14) and euglobal-Ia2 (15). Compounds 6, 7, 10, 12 and 13 had obvious cytotoxic activity on MCF-7 cells with the inhibition rates of 71.83%, 71.11%, 70.00%, 80.53%, and 79.87%, respectively at concentration of 20 μmol/L. The compound 12 showed significant cytotoxic activity against MCF-7 with IC50 value of 13.84 μmol/L. Conclusion Compounds 1-15 are phenolic constituents. Compounds 1 was isolated from Eucalyptus for the first time, compounds 2 and 3 were isolated from this plant for the first time, and compounds 9-15 were isolated from the fruits of E. globulus for the first time. The cytotoxic activity results showed that most of the compounds showed significant anti-MCF-7 activity at a concentration of 20 μmol/L.
[中图分类号]
R284.1
[基金项目]
天津市教委科研计划项目(2019ZD031)