目的 利用三线胶体金侧流向免疫色谱技术，针对中药特点对样品前处理和检测曲线进行研究，建立快速、准确的定量测定中药饮片中黄曲霉毒素B1、B2、G1和G2总量的方法，迅速检测中药饮片中黄曲霉毒素的污染程度。方法 利用胶体金免疫亲和法建立《中国药典》2020年版规定限度的24种中药饮片中黄曲霉毒素测定方法，进行方法学验证，并对60种中药饮片，共计195批次样品进行分类测定。同时利用液质联用（三重四级杆质谱法）对上述样品进行定量检测，并比对2种检测方法。采用配对t检验法比较2种方法的检测结果是否存在显著性差异；通过加入其他真菌毒素对试纸条的特异性进行考察。结果 通过胶体金侧流向免疫色谱技术测定中药饮片中黄曲霉毒素含量，胶体金方法和液质联用方法检测结果一致，无显著性差异，加样回收率为80.3%～119.7%，与黄曲霉毒素M1和M2、赭曲霉毒素、脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮等其他真菌毒素没有交叉反应。单独研究黄曲霉毒素B1与B1、B2、G1和G2总量之间无交叉干扰，且其含量测定符合《中国药典》2020年版规定的标准检验要求。结论 胶体金检测方法可以准确、定量、快速检测中药饮片中黄曲霉毒素含量。该方法具有简单、快捷和低毒等优点。

Objective A rapid and accurate method for quantitative determination of aflatoxins B1, B2, G1, and G2 in Chinese herbal pieces was established by using colloidal gold-side flow immunochromatography, and the pollution level of aflatoxins in prepared slices of Chinese herbal pieces was determined rapidly. Methods The content of aflatoxin in 24 kinds of Chinese herbal pieces was determined by colloidal gold immunoaffinity method, and the results were compared by liquid mass spectrometry. The paired t test was used to compare whether there was significant difference between the two methods. The specificity of the test strip was investigated by adding other fungal toxins. Tonga calculated the recovery rate. Results The aflatoxin content in the Chinese herbal pieces were determined through side to immune colloidal gold chromatography technology. The test results of colloidal gold method and LC-MS/MS method were consistent. There was no significant difference, result in three levels in the low recovery rate was between 80.3% and 119.7%, and aflatoxin M1 and M2, ochre and aspergillus toxin, vomiting toxins and corn gibberellic ketene and other mycotoxins had no cross reaction, which met the requirements. Conclusion Colloidal gold assay can be used for accurate, quantitative and rapid determination of aflatoxins in prepared slices. The method has the advantages of simplicity, rapidity and low toxicity.

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