目的 研究酥脆掘海绵Dysidea fragilis中杂萜类化学成分。方法 采用硅胶柱色谱、反相ODS中压柱色谱以及半制备高效液相色谱等多种色谱技术进行分离纯化，所获得化合物采用HRMS、NMR等波谱方法进行鉴定，绝对构型则通过量子化学计算（ECD）法确定。结果 从酥脆掘海绵的95%乙醇提取物中分离得到了3个杂萜类化合物和1个二萜类化合物，分别鉴定为酥脆内酯（1）、黑麦草内酯（2）、cavernosine（3）和dictyol E（4）。结论 化合物1 为新化合物，首次对化合物2 的核磁数据进行了全归属，纠正了其C-6和C-8位碳信号归属的错误。

Objective To study the chemical constituents of meroterpenoids from the sponge Dysidea fragilis. Methods Silica gel column chromatography, reverse phase ODS medium pressure column chromatography and semi-preparative high performance liquid chromatography were used for separation and purification. The obtained compounds were identified by HRMS, NMR and other spectral methods, and the absolute configuration was determined by comparison of the experimental and calculated ECD spectra. Results Four meroterpenoids were isolated from the 95% ethanol extract of the sponge D. fragilis, which were identified as fragiloid (1), loliolide (2), cavernosine (3), and dictyol E (4). Conclusion Compound 1 is a new compound. The NMR data of compound 2 has been fully assigned for the first time, and the mistake in the assignment of its carbon signal has been corrected.

R284.1

国家自然科学基金资助项目（82022068）；国家自然科学基金资助项目（41576130）