目的 研究大叶铁线莲Clematis heracleifolia全草的化学成分。方法 利用硅胶、Sephadex LH-20凝胶、ODS反相柱色谱和半制备高效液相色谱等分离方法进行分离纯化，结合核磁共振、质谱等波谱学技术鉴定其化学结构。结果 从大叶铁线莲全草75%乙醇提取物的正丁醇萃取部位分离鉴定了10个化合物，包括三萜皂苷6个、黄酮苷1个、木栓烷型三萜2个和植物甾醇1个，分别为3，23-二羟基-齐墩果-18-烯-28-酸-O-α-L-吡喃鼠李糖基-（1→4）-β-D-吡喃葡萄糖基-（1→6）-β-D-吡喃葡萄糖苷（1）、刺楸皂苷B（2）、粗齿铁线莲皂苷B（3）、刺五加苷A₁（4）、皂苷PJ₃（5）、异株五加苷B（6）、金丝桃苷（7）、木栓酮（8）、表木栓醇（9）和β-谷甾醇（10）。结论 化合物1 是新化合物，命名为大叶铁线莲皂苷D；化合物2 ～9 均为首次从大叶铁线莲中分离得到。

Objective To investigate the chemical constituents from the whole plants of Clematis heracleifolia. Methods The n-BuOH part of 75% ethanol extraction of the whole plants was isolated by slilica gel, Sephadex LH-20, ODS column chromatographies and semi-preparative HPLC. The structures of compounds were identified by NMR, MS, and other spectroscopic techniques. Results Ten compounds, including 3,23-dihydroxyolean-18-en-28-oic acid-O-α-L-rhamnopyransyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside (1), kalopanax saponin B (2), clemargenoside B (3), ciwujianoside A₁(4), saponin PJ₃ (5), sieboldianoside B (6), hyperin (7), friedelin (8), epifriedelinol (9), and β-sitosterol (10) were isolated and identified. Conclusion Compound 1 is a new compound named clemahergenoside D, and compounds 2-9 are isolated from the whole plants of C. heracleifolia for the first time.

R284.1

国家自然科学基金资助项目（81274029）