目的 分析漆酶在唇形科药用植物中的异同，以期为唇形科药用植物中漆酶的后续研究提供依据。方法 通过生物信息学方法从唇形科8种药用植物的转录组数据库中发掘了31个完整漆酶基因并解析其特征，包括序列的同源性、理化特征、信号肽、导肽、跨膜结构域、糖基化与磷酸化位点、二级结构和铜离子结构域等，并对它们的进化关系以及表达模式进行了初步分析。结果 唇形科药用植物漆酶基因均具有典型的漆酶结构特征，相对分子质量在62 000左右，多数含有分泌途径的信号肽，无规则卷曲和延伸链是其二级结构的主要构成部分；通过与拟南芥Arabidopsis thaliana和丹参Salvia miltiorrhiza漆酶共同构建系统进化树，能将其分为7个亚族。结论 对唇形科药用植物漆酶基因的详细分析，可为后续该酶基因的深入研究以及通过生物技术手段对唇形科药用植物药效物质进行代谢调控以及关键酶基因的定点突变提供参考信息。

Objective To provide useful information for the further study of Labiatae laccases through sequence comparison among eight Labiatae medicinal plants. Methods Thirty-one full-length laccase genes were found from the transcriptome database of eight medicinal plants of Labiatae by bioinformatics method and their characteristics were analyzed, including sequence homology, physicochemical characteristics, signal peptides, target peptides, transmembrane helices, glycosylation and phosphorylation sites, secondary structure and Cu-oxidase domains. Their evolutionary relationships and expression patterns were also analyzed. Results The laccase genes of medicinal plants in Labiatae have typical laccase structures. Their molecular weight was about 62 000, and most of them contain signal peptides. Random coils and extended strands are the main components of the secondary structure of laccases. The Labiatae laccases can be divided into seven subfamilies by constructing a phylogenetic tree with laccases of Arabidopsis thaliana and Salvia miltiorrhiza. Conclusion The detailed analysis of laccase genes in medicinal plants of Labiatae in this study will provide important information for further study of these genes as well as the regulation of the secondary metabolites of these medicinal plants through laccase mutation.

R282.12

国家自然科学基金资助项目（31770329）；国家自然科学基金资助项目（32070327）；国家重点研发计划资助项目（2019YFC1711100）；中国博士后科学基金资助项目（2019M661602）；泉州医学高等专科学校重点科技项目（XJK1601A）