目的 基于网络药理学探讨山花晶颗粒抗视疲劳的作用机制。方法 利用中药系统药理学分析平台（TCMSP）和中药分子机制的生物信息学分析工具（BATMAN）筛选山花晶颗粒的活性成分和潜在作用靶点，通过STRING数据库富集信号通路；采用Cytoscape软件分析蛋白质-蛋白质相互作用（protein-protein interaction，PPI）网络中的关键靶点，采用分子对接实验对主要活性成分和关键靶点的结合能力进行验证。结果 山花晶颗粒筛选出槲皮素、熊果酸、大黄素等66个主要活性成分以及多巴胺受体D2（dopamine receptor D2，DRD2）、5-羟色胺受体1A（5-hydroxytryptamine receptor 1A，HTR1A）、毒蕈碱型胆碱受体M2（cholinergic receptor muscarinic 2，CHRM2）等34个潜在作用靶点，富集分析出神经活性配体-受体相互作用、钙信号通路、环单磷酸腺苷（cyclic adenosine monophosphate，cAMP）信号通路等35条相关信号通路，分子对接结果表明主要活性成分与关键作用靶点的对接构象合理。结论 山花晶颗粒能够通过“多成分-多靶点-多通路”抑制细胞凋亡和炎性因子的产生，从而减轻眼部细胞氧化和损伤，发挥抗视疲劳的作用。

Objective To investigate the mechanism of Shanhuajing Granules (山花晶颗粒) on reliving visual fatigue based on network pharmacology.Methods TCMSP and BATMAN databases were used to obtain the active components and potential action targets, STRING database was utilized to gain interaction network information. The key targets of protein-protein interaction (PPI) network were analyzed by Cytoscape software, molecular docking was used to identify the binding ability between main active components and key targets. Results A total of 66 main active components such as quercetin, ursolic acid and emodin, 34 potential targets such as dopamine receptor D2 (DRD2), 5-hydroxytryptamine receptor 1 (AHTR1A), cholinergic receptor muscarinic 2 (CHRM2) and 35 related signaling pathways such as neuroactive ligand-receptor interaction, calcium signaling pathway, cyclic adenosine monophosphate (cAMP) signaling pathway were obtained. Molecular docking results showed that the main active components docked into key targets.Conclusion Shanhuajing Granules may inhibits cells apoptosis and production of inflammatory factors, reduce the oxidation and injury of eye cells by "multi-ingredient-multi-target-multi-pathway" mode, thereby relive visual fatigue.

R285.5

国家“重大新药创制”科技重大专项（2018ZX09201010）