目的 根据白术Atractylodis Macrocephalae Rhizoma指纹图谱形状特征，建立一种快速精准辨识不同产地与炮制品的方法。方法 建立45批不同产地与炮制法的白术UPLC指纹图谱库，运用总量统计矩及相似度法、正交偏最小二乘-判别分析（orthogonal partial least squares-discriminant analysis，OPLS-DA）法辨析此指纹图谱的形状特点，捕获其特征指纹图谱及特征峰。结果 生品、麸炒、土炒白术UPLC指纹图谱的共有峰16个；安徽与湖南、安徽与浙江、湖南与浙江产地的生白术的总量统计矩相似度分别为0.774～0.982、0.799～0.969、0.788～0.886；3产地的生品与麸炒、生品与土炒、麸炒与土炒白术的总量统计矩相似度中最大值为0.990，最小值为0.774。再结合OPLS-DA法中变量重要性投影（variable importance for the projection，VIP）分析获得其中1～3、6、16号为区分贡献度最大的5个标志峰，确认6号峰为白术内酯II。结论 这5个标志峰对应物质可认定白术的潜在质量标志物（Q-Marker），为白术的产地、炮制法的精准识别及其质量控制与评价提供理论与实验依据，同时也为中药指纹图谱辨形论质做了积极探索。

Objective To establish a rapid and accurate identification method for different processed products and producing areas based on the fingerprint shape characteristics of Baizhu[Atractylodis Macrocephalae Rhizoma (AMR)]. Methods A UPLC fingerprint database of 45 batches of AMR from different producing areas and processing methods was established. The shape characteristics of the fingerprint were analyzed by presidential statistical moment similarity method and orthogonal partial least squares-discriminant analysis (OPLS-DA) method, and the characteristic fingerprint and characteristic peaks were captured. Results There were 16 common peaks in UPLC fingerprints of AMR, bran-processed AMR, soil-processed AMR; The presidential moment similarity between Anhui and Hunan, Anhui and Zhejiang, Hunan and Zhejiang were 0.774-0.982, 0.799-0.969 and 0.788-0.886, respectively. The maximum and minimum of the presidential moment similarity of crude products and bran-processed, crude products and soil-processed, bran-processed and soil-processed AMR from the three areas were 0.990 and 0.774, respectively. Combined with VIP analysis (variable importance for the projection) by OPLS-DA method, 1-3, 6, 16 peaks were the five marker peaks with the largest contribution to discrimination. Peak 6 was identified as atractylenolide II. Conclusion The corresponding substances of these five marker peaks can be identified as the potential quality markers of AMR, which provides theoretical and experimental basis for accurate identification areas, processing method, quality control and evaluation of AMR, and mades active exploration on the quality of TCM fingerprint.

R283.6

国家自然科学基金资助项目（81874344）；湖南省自然科学基金资助项目（2019JJ40220）；湖南省自然科学基金资助项目（2021JJ30514）；湖南省中管局项目（2021204）；湖南省中管局项目（2021073）；湖南省教育厅项目（19A366）；湖南省教育厅项目（19A382）；湖南省教育厅项目（19C1435）；长沙市科技计划项目（kq1907136）；湖南省一流学科开放基金项目（2018YX11）