目的 建立基于HPLC-UV技术的一测多评法（QAMS）同时测定尖叶假龙胆Gentianella acuta药材中雏菊叶龙胆酮、龙胆苦苷、芒果苷、当药醇苷、木犀草素和去甲基雏菊叶龙胆酮的含量。方法 采用HPLC-UV技术，以雏菊叶龙胆酮为内参物，分别测定其与龙胆苦苷、芒果苷、当药醇苷、木犀草素和去甲基雏菊叶龙胆酮的相对校正因子，并计算含量；通过外标法与QAMS法分别对6种成分含量进行比较，以检验QAMS法的适用性、准确性和可重复性。结果 QAMS法与外标法所测得的结果无显著性差异。结论 所建立的QAMS方法可满足尖叶假龙胆在缺乏对照品的情况下，完成尖叶假龙胆中多指标成分的同时测定，为尖叶假龙胆质量控制标准的建立提供依据。

Objective To detect the contents of six components in Gentianella acuta by establishing the method of quantitative analysis multi-components by single marker (QAMS). Methods Using HPLC-UV technology and taking bellidifolin as internal standard, the relative calibration factor of gentiopicroside, mangiferin, swertianolin, luteolin and demethylbellidifolin were detected respectively. The contents of the six measured components were compared by using the external standard method and the QAMS method, in order to verify the applicability, accuracy and repeatability of QAMS. Results There were no significant differences between QAMS method and external standard. Conclusion The established method can be used to simultaneous detect the multi-index components of G.acuta in the absence of relevant control, which could be helpful to establish the quality control specification of G.acuta.

R286.2

国家自然科学基金项目（81573698）；河北省省属高校基本科研业务费青年教师科研能力提升项目（YJZ2019001）；河北省省属高校基本科研业务费优秀学生科研能力提升项目（YXZ20190005）