目的 建立不同产地当归Angelicae sinensis的HPLC指纹图谱并测定其主要成分的含量，结合化学计量学分析，建立各产地当归的“识别标志”。方法 采用HPLC-DAD方法建立甘肃、四川和云南等地当归的指纹图谱，并对指标成分进行含量测定。进行相似度评价结合主成分分析（principal component analysis，PCA）、偏最小二乘法判别分析（partial leastsquares discrimination analysis，PLS-DA）、Fisher线性判别（fisher linear discrimination analysis，FLDA）等模式识别技术进行分析。结果 HPLC指纹图谱共标定了不同产地当归中的13个共有峰，指认出绿原酸、阿魏酸、洋川芎内酯I、洋川芎内酯H、阿魏酸松柏酯、E-藁本内酯、E-丁烯基苯酞、Z-藁本内酯8个色谱峰，样品相似度均在0.90以上。绿原酸、洋川芎内酯I、E-丁烯基苯酞、总阿魏酸（阿魏酸与阿魏酸松柏酯换算后总量）、Z-藁本内酯6个指标成分含量测定变化范围分别为0.10～0.52 mg/g、0.10～0.24 mg/g、0.19～0.54 mg/g、1.42～2.66 mg/g、21.75～29.15 mg/g。PCA、PLS-DA分析结果表明，Z-藁本内酯、E-藁本内酯、阿魏酸松柏酯、阿魏酸在不同产地当归的中变化显著，Z-藁本内酯、E-丁烯基苯酞、总阿魏酸分别可作为甘肃当归、四川当归、云南剑川当归的成分识别标志。逐步线性判别验证当归产地其中自身判别正确率为96.3%，交叉验证正确率为92.3%。结论 建立的HPLC指纹图谱结合含量测定、PCA、PLS-DA客观、全面、有效地对不同产区当归质量特征进行探究，确认各产地当归的标志性成分，为当归的产地溯源及质量控制提供科技支撑。

Objective To establish fingerprints of Danggui (Angelicae Sinensis Radix, ASR) from different producing areas and determine the contents of its main components, combined with chemometric analysis, to establish "identification marks" of Angelicae Sinensis Radix from different producing areas. Methods The fingerprints of Angelicae Sinensis Radix in Gansu, Sichuan and Yunnan were established by HPLC-DAD method, and the content of index components was determined. On this basis, the similarity evaluation system software (2012 version) combined the principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and fisher linear discrimination analysis (FLDA) and other chemometric methods were used for the pattern recognition research. Results The fingerprints were co-calibrated with 13 common peaks in Angelicae Sinensis Radix from different regions. Eight chromatographic peaks were identified by reference. The 27 batches of Angelicae Sinensis Radix samples had similarities above 0.90; The contents variation ranges of chlorogenic acid, ligustilide I, E-butenylphthalide, total ferulic acid (total amount of ferulic acid and coniferyl ferulate converted), and Z-ligustilide were 0.10—0.52 mg/g, 0.10—0.24 mg/g, 0.19—0.54 mg/g, 1.42—2.66 mg/g, 21.75—29.15 mg/g, respectively. PCA and PLS-DA results showed that Z-ligustilide, E-ligustilide, coniferyl ferulate, and ferulic acid were the different components of ASR from different places of origin, among which Z-ligustilide can be used as the component identification mark of Gansu ASR, E-butenyl phthalide can be used as the component identification mark of Sichuan ASR; total ferulic acid can be used as the component identification mark of Yunnan Jianchuan ASR. The content of the index components was linearly discriminant. The correct rate of self-discrimination was 96.3% and the correct rate of cross-validation was 92.3%. Conclusion The established HPLC fingerprint is combined with content determination, PCA and PLS-DA indicators to comprehensively and effectively explore the quality characteristics of Angelicae Sinensis Radix in different production areas, confirm the iconic ingredients of Angelicae Sinensis Radix from various production areas, and provide technical support for the production area origin and quality control of Angelicae Sinensis Radix.

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