目的 基于指纹图谱及网络药理学对当归四逆汤（Danggui Sini Decoction，DSD）进行全过程质量控制和温经散寒、养血通脉功效关联物质预测分析。方法 建立DSD物质基准对应实物的指纹图谱，匹配共有峰，进行共有峰的归属分析；基于可测性和可追溯性进行DSD活性成分的筛选，采用网络药理学构建“成分-靶点-通路”网络，预测DSD温经散寒、养血通脉功效关联物质。结果 建立了DSD物质基准对应实物的指纹图谱，标定25个共有峰，指认了芍药内酯苷、芍药苷、阿魏酸、甘草苷、肉桂酸、邻甲氧基桂皮醛、甘草酸、藁本内酯、细辛脂素9个色谱峰，相似度均>0.90。根据各共有峰的归属分析，主要药效物质群可以从饮片到物质基准对应实物较为完整的传递，归属关系清晰明确；采用网络药理学对筛选出的9个活性成分进行“成分-靶点-通路”网络构建与分析，富集的通路中包括癌症信号通路、磷脂酰肌醇-3-羟激酶-蛋白激酶B（phosphatidylinositol-3-hydroxykinase-protein kinase B，PI3K-Akt）信号通路、丝裂原活化蛋白激酶（mitogen-activated protein kinase，MAPK）信号通路、瞬时受体电位（transient receptor potential，TRP）通道的炎性介质调节等，DSD的9个活性成分可能通过调节这些信号通路达到温经散寒、养血通脉的作用。结论 建立了经典名方DSD的指纹图谱并对其进行归属分析，明确DSD的色谱峰归属，对经典名方DSD进行整体化、过程化的质量控制；并结合网络药理学发现DSD的9个活性成分具有传递性和溯源性，且与DSD温经散寒、养血通脉功效属性密切相关，可作为其潜在的功效关联物质，为DSD的进一步研究提供依据。

Objective Based on fingerprint and network pharmacology research, the whole process quality control of Danggui Sini Decoction (DSD, 当归四逆汤) was carried out, and the efficacy related substances of warming meridian and dispelling cold, nourishing blood and promoting pulse were predicted and analyzed. Methods The fingerprint of DSD substance standard corresponding substance was established, the common peaks were matched and the attribution of the common peaks were analyzed; The active components of DSD were screened based on the testability and traceability, and used network pharmacology to construct the “component-target-pathway” network to predict the efficacy related substances of DSD for warming meridian and dispelling cold, nourishing blood and promoting pulse. Results The fingerprint of DSD was established, 25 common peaks were identified, and nine peaks were identified, including albiflorin, paeoniflorin, ferulic acid, liquiritin, cinnamic acid, 2-methoxy cinnamal dehyde, glycyrrhizic acid, ligustilide, asarinin, the similarity was all greater than 0.90. According to the attribution analysis of each common peak, the main pharmacodynamic substance groups can be completely transferred from the decoction pieces to the substance standard, and the attribution relationship was cleared; The network pharmacology was used to construct and analyze the “component-target-pathway” network of the nine active ingredients, and the enriched pathways include pathways in cancer, PI3K-Akt signaling pathway, MAPK signaling pathway, inflammatory mediator regulation of TRP and other signaling pathways, the nine active components of DSD may regulate these signaling pathways to achieve the functions of warming meridians, dispersing cold, nourishing blood and promoting pulse. Conclusion In this study, the fingerprint of classical prescription DSD was established and its attribution analysis was carried out to clarify the chromatographic peak attribution of DSD. and the quality control of classical prescription DSD was carried out in an integrated and procedural way. Combined with network pharmacology, it was found that the nine active components of DSD had transmissibility and traceability, and were closely related to the efficacy of DSD in warming meridians and dispelling cold, nourishing blood and promoting pulse, which could be used as potential efficacy related substances to provide basis for the further study of DSD.

R283.6

国家重点研发计划项目（2018YFC1707000）