目的 研究翻白草Potenlilla discolor的化学成分及其α-葡萄糖苷酶抑制活性。方法 以4-硝基苯基-α-吡喃葡萄糖苷（PNPG）为底物进行α-葡萄糖苷酶抑制活性筛选。利用硅胶、聚酰胺、Sephadex LH-20凝胶、ODS及反相HPLC等柱色谱方法进行分离和纯化，并运用核磁共振波谱鉴定化合物的结构。结果 翻白草全草95%乙醇水浸提物的二氯甲烷、醋酸乙酯、正丁醇等萃取物经D101大孔吸附树脂分离的30%乙醇水洗脱部分显示强的α-葡萄糖苷酶抑制活性，对其进一步的化学成分研究共分离得到17个化合物，分别鉴定为3，3'，4，4'-四羟基联苯（1）、反式银椴苷（2）、顺式银椴苷（3）、柚皮素-5-O-β-葡萄糖苷（4）、异杞柳苷（5）、黑色五味子单体苷（6）、紫椴苷B（7）、（-）-异落叶松脂素-9'-O-β-D-吡喃木糖苷（8）、2-羰基-坡模酸（9）、齐墩果酸（10）、熊果酸（11）、2α，3α，19α-三羟基-12-烯-28-熊果酸（12）、2α-羟基齐墩果酸（13）、覆盆子酸（14）、山柰酚（15）、山柰酚-3-O-β-D-葡萄糖醛酸苷（16）、槲皮素-3-O-β-D-葡萄糖醛酸-6''-甲酯（17）。结论 化合物1、3～8均为首次从委陵菜属植物中分离得到，化合物9～17均对α-葡萄糖苷酶有抑制作用。

Objective To study the chemical constituents of Potentilla discolor and their α-glucosidase inhibitory activity. Methods The constituents were isolated and purified by silica gel, polyamide, ODS, Sephadex LH-20, and RP-HPLC column chromatography. Their structures were elucidated by NMR spectra. PNPG method was used to determine the α-glucosidase inhibitory activity. Results Dichloromethane extract, ethyl acetate extract, 30% ethanol elution fraction of n-butanol extract separated by D101 macroporous resin showed potent α-glucosidase inhibitory activity. Further chemical study resulted in the purification of 17 compounds and they were identified as 3,3',4,4'-tetrahydroxybiphenyl (1), trans-tiliroside (2), cis-tiliroside (3), naringenin-5-O-β-glucoside (4), isosalipurposide (5), schizandriside (6), tiliamuroside B (7), (-)-isolariciresinol-9'-O-β-D-xylopyranoside (8), 2-oxo-pomolic acid (9), oleanolic acid (10), ursolic acid (11), 2α,3α,19α-trihydroxy-12-ursen-28-oic acid (12), 2α,3β-dihydroxyolean-12-en-28-oic acid (13), fupenzic acid (14), kaempferol (15), kaempferol-3-O-β-D-glucuronide (16), and quercetin-3-O-β-D-glucuronide-6''-methyl ester (17). Conclusion This is the first report of compounds 1, 3—8 isolated from the genus of Potenlilla. Compounds 9—17 show α-glucosidase inhibitory activity.

R284.1

天津市科技计划项目（20ZXGBSY00050）