[关键词]
[摘要]
目的 对比不同基原忍冬属药材的差异性。方法 UPLC建立忍冬属药材指纹图谱,采用Waters BEH Phenyl(100 mm×2.1 mm,1.7 mm)色谱柱,以乙腈-0.1%磷酸为流动相,梯度洗脱,体积流量0.3 mL/min,柱温30 ℃,检测波长240 nm,建立47批次不同基原忍冬属药材的指纹图谱,同时对10个指标成分进行同法测定,并采用主成分分析(principal component analysis,PCA)和正交偏最小二乘法-判别分析(partial least squares discrimination analysis,OPLS-DA)进行多变量统计分析。结果 建立了忍冬属药材UPLC指纹图谱,其中忍冬确定15个共有峰、灰毡毛忍冬确定8个共有峰、黄褐毛忍冬确定9个共有峰,淡红忍冬确定11个共有峰,聚类分析可将47个样本按基原聚为4类;通过主成分分析可将不同基原的忍冬属药材进行区别;OPLS-DA分析可用于忍冬属的基原识别,并筛选出5个差异性成分。结论 建立的方法简便、快捷、可靠,为不同基原忍冬属药材的质量控制和基原鉴别提供可靠的分析方法。
[Key word]
[Abstract]
Objective Comparison of the differences of different primordial Lonicera Methods The fingerprint of Lonicera was established by UPLC, the chromatographic column was Waters BEH Phenyl chromatographyic column (100 mm×2.1 mm,1.7 μm), and acetonitrile-0.1%phosphoric formic acid solution was used as the mobile phase with the gradient elution, the flow rate was 0.3mL/min, the column temperature was 30 ℃, the detection wavelength was 240 nm. The fingerprints and 10 index components of 47 batches of Lonicera from different origins were determined by the same method. Meanwhile, multivariate analysis was carried out by PCA and OPLS-DA. Results UPLC method was used to establish the fingerprint of Lonicera, There are 15 common peaks in Lonicera japonica Thunb., 8 common peaks in Lonicera macranthoides Hand.-Mazz., 9 common peaks in Lonicera fulvotomentosa Hsu et S.C.Cheng, and 10 common peaks in Lonicera hypoglauca Miq. Cluster analysis was divided 47 samples into four group, at the same time, and the results of principal component scores can distinguish the different origins of Lonicera; besides, OPLS-DA analysis can be used to identify the different origins of Lonicera and screen out five different components. Conclusion The method is simple, efficient and reliable, which can be used for the quality control and identification among different Lonicera medicinal plants.
[中图分类号]
R286.2
[基金项目]
广东省科技计划项目(2018B030323004);东省重点领域研发计划项目(2019B110209005)