[关键词]
[摘要]
目的 基于HPLC指纹图谱、多成分定量与化学模式识别相结合的方法,评价不同产地消瘤藤Pileostegia tomentella质量,为其进一步开发利用提供依据。方法 采用HPLC法,流动相为乙腈-0.1%磷酸水溶液进行梯度洗脱,检测波长为246、265、320 nm,对14批不同产地消瘤藤药材进行指纹图谱及5种有效成分含量测定研究,应用聚类分析与主成分分析等化学模式识别法对消瘤藤质量进行评价。结果 14批消瘤藤的指纹图谱相似度均在0.875以上,茵芋苷、紫丁香苷、当药苷、7-羟基香豆素和7-羟基-8-甲氧基香豆素质量分数分别为1.280 5~9.431 6、0.001 5~0.1558、0.089 8~1.863 1、0.328 9~3.382 6、0.073 9~1.112 8 mg/g;选取了12个色谱峰作为指纹图谱的共有峰,通过聚类分析可将14批消瘤藤分为3类,主成分分析与聚类分析结果基本一致。通过主成分分析,进一步评价不同产地间质量的差异,从而发现S1、S12、S14等地的质量较优。结论 广西不同产地消瘤藤存在一定质量差异。指纹图谱与含量测定相结合,应用化学模式识别法可全面评价消瘤藤质量。该法的建立为消瘤藤的质量控制及评价提供依据。
[Key word]
[Abstract]
Objective Based on the method of HPLC fingerprint, multi-component quantification and chemical pattern recognition, the quality of Pileostegia tomentella from different producing areas was evaluated to provide basis for further development and utilization. Methods The HPLC using acetonitrile-0.1% phosphoric acid aqueous solution as mobile phase based on the wavelength (246 nm, 265 nm and 320 nm) and gradient elution method was developed to evaluate the quality of 14 batches of Pileostegia tomentella from different producing areas. The combination of chromatographic fingerprints and quality determination of five active ingredients, as well as chemometrics including hierarchical cluster analysis and principal component analysis, were further employed for the quality assessment. Results The fingerprint similarity of 14 bathes of Pileostegia tomentella were all above 0.875. The content rangs of skimming, syringing, sweroside, 7-hydroxycoumarin and 7-hydroxy-8-methoxycoumarin were 1.280 5—9.431 6 mg/g, 0.001 5—0.155 8 mg/g, 0.089 8—1.863 1 mg/g, 0.328 9—3.382 6 mg/g, and 0.073 9—1.112 8 mg/g. A total of 12 chromatographic peaks were selected as the common peaks of the fingerprint, 14 batches of Pileostegia tomentella could be divided into three categories by cluster analysis, and the results of principal component analysis and cluster analysis were basically the same. principal component analysis was used further evaluate the quality differences of the samples from different producing areas, and it was found that the medicinal materials from S1, S12 and S14 were of superior quality. Conclusion The quality of Pileostegia tomentella from different producing areas of Guangxi was different to a certain extent. By combination of fingerpint and content determination, the chemical pattern recognition method can be used to evaluate the quality of the Pileostegia tomentella comprehensively. The establishment of this method provide a reference for the quality control and evaluation of Pileostegia tomentella.
[中图分类号]
R286
[基金项目]
广西科技计划项目(桂科AD17195002);广西自然科学基金面上项目(2020GXNSFAA259067);广西中医药适宜技术开发与推广项目(GZSY20-04);广西中药质量标准研究重点实验室自主研究课题(桂中重自201705)