[关键词]
[摘要]
目的 通过药效学实验与正交试验相结合的方式,确定消疹止痛凝胶的提取工艺路线及工艺参数。方法 选用单纯疱疹病毒致豚鼠皮肤感染模型和热板致小鼠疼痛模型进行初步药效学实验,比较乙醇渗漉、乙醇回流、水煎煮3种提取方法所得提取液对疱疹症状的减轻程度和止痛作用的差异,筛选提取工艺路线;再通过正交试验,以延胡索乙素转移率和干膏得率为指标对乙醇渗漉法的提取工艺参数进行优化。结果 药效学实验结果显示,乙醇渗漉法所得的提取物对豚鼠皮肤疱疹病毒有明显减轻作用,并可缩短痊愈时间;在给药后0.5、1、2 h均能够显著延长小鼠的痛阈值,表现出明显的止痛作用,因此,确定为消疹止痛凝胶的提取工艺路线;进一步通过正交试验优化得到的提取工艺为以60%乙醇渗漉提取,渗漉体积流量为4 mL/min,收集渗漉液体积为8倍药材量。结论 所得的提取工艺合理、可行,为后期消疹止痛凝胶的制剂工艺研究奠定基础。
[Key word]
[Abstract]
Objective Through the combination of pharmacodynamic test and orthogonal test, the extraction technology route and parameters of Xiaozhen Zhitong Gel (XZG, 消疹止痛凝胶) were optimized. Methods The skin infection model of guinea pigs induced by herpes simplex virus and the pain model of mice induced by hot plate were chosen to perform the preliminary pharmacodynamic test. The effects of extracts from three technology routes of ethanol percolation, ethanol reflux, and water decoction on alleviation herpes symptoms and pain were compared to ascertain the extraction technology route. Then the extraction technology parameters of ethanol percolation method were optimized by orthogonal test with the transfer rate of tetrahydropalmatine and dry extract yield as indexes. Results The results of pharmacodynamics test showed that the extracts obtained by ethanol percolation method could obviously alleviate guinea pig skin herpesvirus and shorten the healing time. Besides, the pain threshold of mice was also significantly prolonged after administering the extracts for 0.5 h, 1 h, and 2 h, demonstrating the obvious analgesic effect. Therefore, the ethanol percolation method was ascertained as the extraction technology route of XZG. The extraction technology parameters were further optimized by orthogonal test as follows: 60% ethanol was used for percolation extraction, the percolation rate was 4 mL/min, and the volume of percolation solution was collected up to 8 times of the medicinal materials amount. Conclusion The obtained extraction technology is reasonable and feasible, which will lay the foundation for the pharmaceutical technology of XZG in the future research.
[中图分类号]
R283.6
[基金项目]
上海市科委“生物医药领域”科技支撑项目(18401930800);上海市科委优秀学术带头人资助项目(19XD1423700)