目的 以射干Belamcanda chinensis为材料，在测序、组装获得叶绿体基因组的基础上，明确其结构、序列特征及系统发育关系。方法 利用PE150双末端策略进行建库测序，用NOVOPlasty组装完整的叶绿体基因组，经PCR验证边界，借助生物信息学工具进行序列分析和系统发育研究。结果 射干的叶绿体基因组全长为153 816 bp，大单拷贝区、反向重复区和小单拷贝区的长度分别为83 143、26 214、18 245 bp。射干叶绿体基因组共有133个基因，编码基因、tRNA和rRNA的数量分别为92、38和8；ycf1有2个拷贝，其中一个为假基因。系统发育分析结果表明，7种植物叶绿体基因组在发育树上可分为4组，射干与同为鸢尾科的溪荪聚为一组，支持率达100%。结论 射干叶绿体基因组的组装、序列分析和系统发育分析，为该药用植物的遗传结构和遗传多样性研究奠定了基础。

Objective To confirm the genome structure, sequence characteristics, and phylogenetic relationship by sequencing and assembling the chloroplast genome of a medicinal plant Belamcanda chinensis. Methods A PE150 strategy was applied to construct library. The complete chloroplast genome was generated using NOVOPlasty, followed by PCR confirmation of the borders, and sequence analysis, as well as phylogenetic study was conducted by bioinformatic tools. Results The full-length chloroplast genome was 153 816 bp in length, with a large single copy of 83 143 bp, an inverted repeat of 26 214 bp, and a small single copy of 18 245 bp. The B. chinensis chloroplast genome consisted of 133 genes, including 92 protein-coding genes, 38 tRNAs, and 8 rRNAs, respectively. There were two ycf1 genes, one of which was a pseudogene. Phylogenetic evolution analysis results indicated that the seven chloroplast genomes can be divided into four groups, B. chinensis and Iris sanguine from Iridaceae were found to cluster in the same clade, with a support rate of 100%. Conclusion Assembly, sequence analysis and phylogenetic evolution of B. chinensis chloroplast genome provides an insight into studies on both genetic structure and genetic diversity.

R282.12

台州市211人才工程经费资助（2012年度）