目的 定性分析隐绿原酸在大鼠血浆、尿液和粪便中的代谢产物。方法 大鼠给予隐绿原酸后，采集血液、尿液和粪便并进行样品处理，采用UHPLC-Q-TOF/MS法检测各生物样品中的代谢产物。结果 通过相应的色谱保留行为、精确相对分子质量、特征碎片离子、质谱裂解规律、文献报道以及对照品比对，最终分析鉴定了47个代谢产物。其主要体内代谢途径为水解反应、异构化反应、甲基化、氢化、葡萄糖醛酸化以及它们的复合反应。结论 UHPLC-Q-TOF/MS法能较全面地鉴定隐绿原酸在大鼠体内的代谢产物，有助于进一步阐明隐绿原酸的药效学物质基础。

Objective To elucidate the in vivo metabolites of cryptochlorogenic acid in rat plasma, urine and feces samples. Methods Urine, plasma and feces samples were collected after oral gavage. After processing biological sample by solid-phase extraction, an efficient method of ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF) mass spectrometer was established to identify its metabolites. Results According to the obtained accurate mass weight, chromatographic retention behavior, mass spectrometry cleavage, characteristic fragment ions, reference comparison, and related literature reports, a total of 47 metabolites were finally screened and characterized. The main metabolic pathways contained hydrolysis, isomerization, hydrogenation, glucosylation and their complicated reactions. Conclusion The results showed that the metabolism of cryptochlorogenic acid could be comprehensively clarified by using UHPLC-Q-TOF mass spectrometer. Moreover, our study provided the scientific evidence for the further study of the pharmacological material basis of cryptochlorogenic acid.

R284.1

2017年北京市科技新星与领军人才培养专项计划（Z171100001117029）；滨州医学院高层次人才科研启动基金项目（2019KYQD05）；山东省青创人才引育创新研究团队；烟台市校地融合中药大健康产业化平台项目