目的 建立基于UPLC-Q-Exactive-MS/MS法初步筛选彝药材西南委陵菜Potentilla fulgens质量标志物、指纹图谱结合多元统计分析综合评价其药材质量的研究方法，为西南委陵菜质量评价和质量控制提供依据。方法 采用UPLC-Q-Exactive-MS/MS法定性分析西南委陵菜化学成分组成，筛选质量标志物；HPLC法建立27批次西南委陵菜药材指纹图谱，定量分析质量标志物的含量；采用主成分分析和TOPSIS分析综合评价西南委陵菜药材质量。结果UPLC-Q-Exactive-MS/MS法从西南委陵菜中指认了48个化合物，包括原花青素类34个、黄酮类12个、酚类1个、鞣质类1个，筛选出含量较高、可准确测定的表儿茶素作为质量标志物，西南委陵菜指纹图谱中确定了10个共有峰，含量测定结果表明不同产地药材中表儿茶素的含量有较大差异，表儿茶素含量与海拔高度呈正相关，综合主成分分析结合TOPSIS分析，结果表明样品S11的质量最佳，S9的质量最次。结论 指纹图谱结合多元统计分析的药材质量综合评价方法，可较客观评价西南委陵菜药材质量，为其药材的质量控制提供科学依据。

Objective To establish a method for discovering quality marker of herb Potentilla fulgens, and quality evaluation by fingerprint combined with multivariate statistical analysis for its quality control. Methods UPLC-Q-Exactive-MS/MS was used to qualitatively analyze the chemical constituents and screen quality marker. HPLC method was used to establish fingerprint chromatograms of 27 batches of P. fulgens and quantitatively analyze the content of quality marker. Principal component analysis (PCA) and TOPSIS analysis were used to comprehensively evaluate the quality of P. fulgens. Results Total 48 compounds were identified by UPLC-Q-Exactive-MS/MS, including 34 proanthocyanidins, 12 flavonoids, one phenolic, and one tannin. Epicatechin with high content and accurate content determination was selected as quality marker. P. fulgens fingerprint identified 10 common peaks. Epicatechin content of P. fulgens was difference among different origin, and it is positively related with altitude. The results of PCA and TOPSIS analysis showed that the quality of sample S11 was the best, and the quality of sample S9 was the least. Conclusion The comprehensive quality evaluation method of fingerprint combined with multivariate statistical analysis could be used to objectively evaluate the quality of P. fulgens, and provide scientific basis for the quality control of the medicinal materials.

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国家重点研发计划项目（2018YFC1708005）；四川省科技农村领域重点研发（2019YFN0089）；中央高校基本科研业务费专项资金研究类项目（2021PTJS35）