目的 系统阐明灯盏生脉胶囊中的化学成分组成，并初步探究其治疗心绞痛的作用机制。方法 采用超高效液相色谱-四级杆/静电场轨道阱高分辨质谱（UHPLC-Q-Orbitrap HRMS）技术对灯盏生脉胶囊中的化合物进行准确定性，并结合网络药理学分析在灯盏生脉胶囊中治疗心绞痛过程中起调控作用的靶点及通路。结果 共鉴定出黄酮类、木脂素类、酚酸类、皂苷类和其他类共5类共30种化合物，其中16种化合物为灯盏生脉胶囊入血成分，可通过调控丝裂原活化蛋白激酶（mitogen-activated protein kinases，MAPK）通路和p53通路等来发挥治疗心绞痛的作用。结论 为探索灯盏生脉胶囊药效物质基础提供了新的思路，并为指导临床合理用药提供了科学的理论依据。

Objective To illuminate the chemical components from Dengzhan Shengmai Capsules (灯盏生脉胶囊), and reveal its mechanism for treatment of angina pectoris. Methods An ultra-high performance liquid chromatography-quadrupole/orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) method was employed for the qualitative analysis of components in Dengzhan Shengmai Capsules, and network pharmacology was applied for the targets and pathways study of Dengzhan Shengmai Capsules for angina pectoris. Results Thirty compounds in total were successfully identified in this research, including flavones, lignans, phenolic acids, saponins and other categories. Among these compounds, 16 compounds were verified as components in plasma and might play a therapeutic role on angina pectoris through MAPK signaling pathway, p53 signaling pathway and etc. Conclusion This research offers a new method for the pharmacodynamics material basis studies of Dengzhan Shengmai Capsules, which provides scientific theory basis for its clinical rational drug use.

R284.1

国家自然科学基金资助项目（81703666）