目的 研究玉米须的化学成分及其细胞毒、葡萄糖消耗活性。方法 采用溶剂萃取、硅胶柱色谱、高效液相色谱等方法进行分离纯化，通过理化性质及波谱数据分析鉴定结构，采用计算电子圆二色谱（ECD）方法确定新化合物的绝对构型；对分离得到的化合物1、2进行体外细胞毒及葡萄糖消耗活性筛选。结果 从玉米须甲醇浸提液醋酸乙酯萃取物中分离得到4个化合物，分别鉴定为6β，12β-二羟基-4（5），7（8）-玉米二烯（1）、8α，10α-二羟基-4（5），7（12）-玉米二烯（2）、环己甲酸甲酯（3）、氮乙酰对羟基苯乙胺（4）。化合物1对人卵巢癌SK-OV-3细胞及膀胱癌T-24细胞的抑制率分别为11.86%、18.64%，对人肝癌HepG2细胞中葡萄糖消耗促进率为10.30%。结论 化合物1、2是未见报道的新化合物，化合物1对SK-OV-3及T-24细胞显示抑制活性，对HepG2细胞中葡萄糖消耗具有促进作用。

Objective To study the chemical constituents from style and stigma of Zea mays and evaluate its cytotoxic and glucose consumption activities. Methods The chemical constituents were isolated by silica gel column chromatography and HPLC, and its structure were identified by their spectral data and physicochemical properties analysis and the absolute configuration of new compounds was identified by calculating ECD. Compounds 1 and 2 were tested for their effects on cytotoxic and glucose consumption. Results Four compounds were isolated from methanol extract ethyl acetate extracts of style and stigma of Zea mays with the structures identified as 6β,12β-dihydroxy-4(5),7(8)-stigmdiene (1), 8α,10α-dihydroxy-4(5),7(12)-stigmdiene (2), methyl cyclohexane-carboxylate (3), and N-acethyl-tyramine (4). Compound 1 exhibited inhibitory activities on SK-OV-3 and T-24 cells with values of 11.86% and 18.64%. Compound 1 improved the glucose consumption with values of 10.30% in HepG2 cells. Conclusion Compounds 1 and 2 are new compounds. Compound 1 can exhibit inhibitory activities in SK-OV-3 and T-24 cells, which can also improve the glucose consumption in HepG2 cells.

R284.1

黑龙江省属高等学校基本科研业务费项目（YSTSXK201808）