目的 观察防风Saposhnikovia divaricata不同提取部位的燥性，考察其对胶原诱导性关节炎（collagen-induced arthritis，CIA）大鼠滑膜水通道蛋白（aquaporin，AQP）的影响。方法 以正常大鼠为对象，选择饮水量、尿量、粪便含水量、环磷酸腺苷（cyclic adenosine monophosphate，cAMP）含量、环磷酸鸟苷（cyclic guanosine monophosphate，cGMP）含量、血液流变学及不同组织中AQP的表达水平为指标，观察防风不同提取部位的燥性效应；采用CIA大鼠模型，通过测定关节肿胀度、炎症因子含量及关节滑膜AQP-1的表达水平，评价防风不同提取部位对CIA大鼠的影响。结果 防风石油醚部位明显增加正常大鼠的饮水量、尿量，降低粪便含水量，升高血清中cAMP含量和全血黏度，降低肾组织中AQP-2、颌下腺组织中AQP-5和结肠中AQP-9的表达水平（P<0.05、0.01）；防风石油醚部位、正丁醇部位和总提取物能明显降低关节炎大鼠的足肿胀程度，减少血清中白细胞介素-1β（interleukin-1β，IL-1β）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）和前列腺素E₂（prostaglandin E₂，PGE₂）含量，降低滑膜组织中AQP-1的表达水平（P<0.05、0.01）。结论 防风石油醚部位具有一定的燥性和抗关节炎作用，防风总提取物具有较好的抗关节炎作用，但未见明显的燥性效应，其机制可能与防风不同部位对不同亚型的AQP差异调控有关。

Objective To observe the dryness of different extracts of Saposhnikovia divaricata, and investigate their effect on synovial aquaporin (AQP) in collagen-induced arthritis (CIA) rats. Methods The normal rats were given with different extracts of S. divaricata, the amount of drinking water, urine volume, fecal water content, cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP) content, hemorheology and the expression level of aquaporin in different tissues were selected as indicators to observe the dryness effects of different extraction of S. divaricata. The rat model of collagen-induced arthritis CIA was successfully established, the degree of paw swelling, the contents of inflammatory factors and the expression of AQP-1 in synovial were measured to evaluate the effects of different extracts of S. divaricata in collagen-induced arthritis rats. Results The petroleum ether extracts of S. divaricata significantly increased the water intake, urine volume, reduced the fecal water content, increased serum cAMP content and blood viscosity, decreased the expression level of AQP-2 in renal, AQP-5 in submandibular gland and AQP-9 in colon in normal rats (P < 0.05, 0.01). The petroleum ether extracts and total extract of S. divaricata significantly reduced the degree of paw swelling and the contents of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E₂(PGE₂) in serum, and decreased the expression level of AQP-1 in synovial tissue of arthritic rats (P < 0.05, 0.01). Conclusion The petroleum ether extracts of S. divaricata showed dryness and anti-arthritis effects. The total extract of S. divaricata have anti-arthritis effects, but no obvious dryness effects, which may be related to the regulation of different subtypes of AQP of different extracts of S. divaricata.

R285

国家自然科学基金面上项目（81873022）；河南省中医药科研重大专项（2019ZYZD09）