目的 对三尖杉Cephalotaxus fortunei茎叶中的化学成分进行研究。方法 运用正相硅胶柱色谱、ODS反相柱色谱、制备高效液相色谱等分离手段对三尖杉茎叶75%乙醇提取物二氯甲烷萃取部位的化学成分进行分离，采用核磁、质谱等多种波谱技术鉴定化合物结构。结果 从三尖杉茎叶75%乙醇提取物的二氯甲烷萃取部位中分离得到了10个二萜类化合物，包括7个松香烷类二萜、1个海松烷类二萜和2个环庚三烯酮降二萜类化合物；得到的化合物分别鉴定为12，16-二羟基松香烷-6，8，11，13-四烯-3-酮（1）、桧醇（2）、柳杉酚（3）、margoclin （4）、cephafortoid B （5）、torreyayunnin （6）、贡山三尖杉二萜D （7）、6α-羟基山达海松酸（8）、海南粗榧内酯（9）、海南粗榧内酯醇（10）。结论 化合物1为1个新化合物，化合物4、8为首次从三尖杉属植物中分离得到。

Objective To study the chemical constituents from the stems and leaves of Cephalotaxus fortunei. Methods The dichloromethane-soluble part of 75% ethanol extract from the stems and leaves of C. fortunei was isolated by silica gel, ODS column chromatography and preparative high performance liquid chromatography and other separation techniques. The structures of the compounds were identified by NMR, MS, and other spectroscopic techniques. Results Ten diterpenoids, including seven abietane diterpenoids, one pimarane diterpenoid, and two tropone norterpenoids, were isolated from the stems and leaves of C. fortunei, and identified as 12,16-dihydroxyabieta-6,8,11,13-tetraene-3-one (1), hinokiol (2), sugiol (3), margoclin (4), cephafortoid B (5), torreyayunnin (6), lanceolatin D (7), 6α-hydroxy sandaracopimaric acid (8), hainanolide (9), and hainanolidol (10). Conclusion Compound 1 is a new compound, and compounds 4 and 8 are isolated from genus Cephalotaxus for the first time.

R284.1

国家科技重大专项（2018ZX09735005）