目的 研究不同质量浓度的生长素吲哚-3乙酸（indole-3-acetic acid，IAA）及施加方式对丹参Salvia miltiorrhiza根系形态和有效成分积累的影响。方法 IAA设置0、5、10、20、30 mg/L 5个质量浓度，分别采用叶面喷施和根部浇灌的方法处理丹参植株，测定各处理组中丹参根系形态学指标及酚酸类成分和丹参酮类成分的含量。结果 IAA处理总体上促进了丹参侧根的发育和丹参酮类成分的积累。2种施加方式相比，叶面喷施更有利于酚酸类成分含量的提高。结论 外源施加IAA对丹参根系发育和有效成分的合成积累均具有调控作用，进一步深入探究IAA对3个生理过程的作用机制具有重要的理论和实践意义。

Objective To investigate the effects of exogenous auxin (IAA) and application methods on the root morphology and accumulation of bioactive compounds in Salvia miltiorrhiza. Methods S. miltiorrhiza plantlets were treated with IAA by foliar spraying and soil drenching methods, respectively. The concentrations of IAA used in this study were as follows:0 mg/L, 5 mg/L, 10 mg/L, 20 mg/L and 30 mg/L. And the parameters of root morphology, phenolic acids content and tanshinones content were determined after the treatments. Results IAA treatments promoted the lateral root development and enhanced the accumulation of tanshinones of S. miltiorrhiza on the whole. Compared with soil drenching, the foliar spraying method was more efficient to stimulate the accumulation of phenolic acids. Conclusion Exogenous IAA regulated the lateral root development and bioactive compounds accumulation of S. miltiorrhiza simultaneously. It is of great theoretical and practical significance to further explore the regulatory mechanism of IAA on these three physiological processes.

R282.2

国家自然科学基金项目（31700257）；中央本级重大增减支项目“名贵中药资源可持续利用能力建设项目”（2060302）；江苏省高等学校大学生创新创业训练计划项目（201811117096X）