目的 采用代谢组学方法考察补阴药（百合、天冬、麦冬）对正常大鼠能量代谢及相关标志物表达的影响。方法 SD大鼠ig百合、天冬、麦冬水煎液，1次/d，连续21 d，收集尿液和肝脏。采用超高效液相色谱与四极杆-静电场轨道阱-线性离子阱三合一组合式质谱仪（UHPLC-Orbitrap-MS）对大鼠尿液样品进行数据采集；基于多元统计分析，建立主成分分析法（principal component analysis，PCA）和正交偏最小二乘判别分析法（orthogonal partial least squares-discriminant analysis，OPLS-DA）模型，并采用Compound Discoverer 3.1软件通过对照品、mzCloud、人类代谢组学数据库（human metabolome database，HMDB）、京都基因与基因组百科全书（Kyoto encyclopedia of genes and genomes，KEGG）等数据库对筛选的代谢物进行鉴定及通路分析；结合通路分析结果选择与物质代谢、能量代谢相关的关键酶进行实验验证。结果 与对照组相比，补阴药组大鼠尿液中鉴定出共有差异代谢物26个，表达趋势一致的差异代谢物有16个，其中15个差异代谢物显著下调，补阴药抑制糖代谢、脂肪酸代谢、氨基酸代谢、核苷酸代谢等11条能量代谢相关通路。补阴药能够下调糖代谢、能量代谢相关酶活性，上调脂代谢相关酶活性，从而抑制物质代谢、能量代谢。结论 补阴药能够通过下调正常大鼠体内与物质代谢、能量代谢相关代谢物及代谢通路，从而发挥抑制物质代谢、能量代谢的作用。

Objective To investigate the effect of yin-tonifying herbs (Lilii Bulbus, Asparagi Radix, and Ophiopogonis Radix) on energy metabolism and expression of metabolites in normal rats. Methods SD rats were ig Lilii Bulbus decoction, Asparagi Radix decoction and Ophiopogonis Radix decoction, once a day for 21 d, urine and liver were collected. Each group data of urine samples was collected by UHPLC-Orbitrap-MS. The high-throughput metabolome data of the urine of rats based on statistical analysis methods such as principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) was obtained to discover the differential metabolites and metabolic pathways through Compound Discoverer 3.1 software with standards and databases such as mzCloud, human metabolome database (HMDB) and Kyoto encyclopedia of genes and genomes (KEGG). Key enzymes related to material metabolism and energy metabolism were selected for verification based on results of pathway analysis. Results Compared with control group, a total of 26 differential metabolites including 16 metabolites with the same expression trend were identified and 15 metabolites were significantly down-regulated, which inhibited 11 metabolic pathways such as glucose metabolism, fatty acid metabolism, amino acid metabolism, and nucleotide metabolism. Yin-tonifying herbs inhibited material metabolism and energy metabolism through down-regulating expression of enzymes related to glucose metabolism and energy metabolism in liver, and up-regulating expression of enzymes related to lipid metabolism. Conclusion Yin-tonifying herbs show a tendency to inhibit substance metabolism and energy metabolism of normal rats through down-regulating related biomarkers.

R285.1;285.5

国家重点研发计划项目（2018YFC1707100）