目的 研究花椒Zanthoxylum bungeanum正丁醇部位化学成分及其抗氧化活性。方法 采用多种柱色谱和高效液相色谱（HPLC）等进行分离纯化，利用NMR等多种波谱技术鉴定化合物结构；运用ABTS⁺和DPPH自由基清除法对所得化合物进行抗氧化活性评价。结果 从花椒正丁醇部位分离得到1个新化合物和13个已知化合物，分别鉴定为4-O-（3'，4'-亚甲二氧基肉桂酰基）-肉桂酸甲酯（1）、苔黑酚（2）、1-甲氧基-7R，8S-二羟基苯丙醇（3）、咖啡酸甲酯（4）、没食子甲酯（5）、3-甲基2-烯丁醇-1-葡萄糖苷（6）、腺苷（7）、紫花前胡苷（8）、blumenol C glucoside（9）、（6R，9S）-3-氧-α-紫罗兰醇-β-D-吡喃葡萄糖苷（10）、长寿花糖苷（11）、丁香树脂酚-4'-O-β-D-吡喃葡萄糖苷（12）、（+）-南烛木树脂酚（13）、异落叶松脂素（14）。ABTS⁺和DPPH自由基清除实验表明，化合物2、4、5和12~14具有抗氧化活性，其中化合物13和14的ABTS⁺自由基清除活性半数抑制浓度（IC₅₀）分别为5.7和11.0 μmol/L，与阳性对照维生素C（Vc）的IC₅₀（6.4 μmol/L）相当。化合物4、5和13的DPPH自由基清除活性IC₅₀分别为61.2、19.8和101.0 μmol/L，与阳性对照Vc的IC₅₀（53.0 μmol/L）相当。结论 化合物1为1个新化合物，命名为花椒酚A；其中化合物1~5、7~10和14首次从花椒属植物中分离得到，化合物12首次从花椒中分离得到。化合物2、4、5和12~14具有一定的抗氧化活性。

Objective To study the constituents of n-butanol portion of Zanthoxylum bungeanum pericarps and their anti-oxidative effects. Methods A variety of column chromatography and HPLC were used for separation and purification, and the structure of the compounds was identified by NMR and other spectroscopy techniques. ABTS⁺ and DPPH radical scavenging methods were used to evaluate the antioxidant activity of the compounds. Results One new compound (1) and 13 known compounds (2-14) were isolated from n-butanol portion of Z. bungeanum pericarps. They were identified as methyl 4-O-(3',4'-methylenedioxy-cinnamoyl)-cinnamate (1), orcinol (2), 1-methoxy-7R,8S-dihydroxyl-phenylpropanol (3), methylcaffeate (4), methylgallate (5), 3-methyl-but-2-en-1-yl-β-D-glucopyranoside (6), adenosine (7), marmesinin (8), blumenol C glucoside (9), (6R,9S)-3-oxo-α-ionol-β-D-glucopyranoside (10), roseoside (11), syringaresinol-4'-β-D-glucoside (12), (+)-lyoniresinol (13), and isolariciresinol (14). ABTS⁺ and DPPH radical scavenging experiments showed that compounds 2, 4, 5, and 12-14 had antioxidant activity. The IC₅₀of ABTS⁺ radical scavenging activities of 13 and 14 were 5.7 and 11.0 μmol/L, respectively, which were equivalent to the IC₅₀ of positive control (Vc=6.4 μmol/L), and the IC₅₀ of DPPH radical scavenging activities of 4, 5 and 13 were 61.2, 19.8, and 101.0 μmol/L, respectively, which were equivalent to the IC₅₀ of Vc=53.0 μmol/L. Conclusion Compound 1 is a new compound named as bungphenol A. Compounds 1-5, 7-10, and 14 are isolated from the genus Zanthoxylum for the first time, and compound 12 is isolated from Z. bungeanum for the first time. Compounds 2, 4, 5, and 12-14 show antioxidant activities.

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国家自然科学基金资助项目（81673325）；甘肃省自然科学基金资助项目（20JR10RA061，20JR5RA570）；烟台中科先进材料与绿色化工产业技术研究院科技创新项目（AMGCE020）；中国科学院百人计划人才项目