目的 基于UPLC指纹图谱、质量标志物“五原则”和网络药理学，研究道地产区不同产地广藿香药材，预测广藿香治疗病毒感染和胃溃疡的潜在质量标志物。方法 建立广藿香UPLC指纹图谱，利用SIMCA-P 14.1分析软件对14批药材共有峰进行聚类分析，利用偏最小二乘法-判别分析（partial least squares-discrimination analysis，PLS-DA）筛选共有峰组间主要差异成分。基于质量标志物“五原则”，对差异成分进行初步分析，进一步采用网络药理学，通过相应数据库检索差异成分、病毒感染和胃溃疡疾病靶点。采用David 6.8数据库对共有靶点进行基因本体（gene ontology，GO）功能富集分析和京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）通路富集分析，同时构建“成分-靶点-疾病-通路”网络，分析广藿香主要活性成分和关键靶点。结果 UPLC指纹图谱研究结合聚类分析结果显示，广东省2个产地广藿香药材存在一定差异。经PLS-DA分析，筛选出4个差异成分，依次为毛蕊花糖苷、广藿香酮、紫葳新苷和列当苷。根据质量标志物“五原则”结合网络药理学分析结果，预测广藿香主要活性成分为广藿香酮和毛蕊花糖苷，两者主要通过作用于ERBB2、EGFR、TLR4、AKT1、TNF靶点，调控TLR、ErbB、MAPK等信号通路，发挥抗病毒、调节肠胃功能等作用。结论 建立的UPLC指纹图谱方法简便，筛选的2个活性成分毛蕊花糖苷和广藿香酮可作为广藿香潜在的质量标志物，为广藿香药材质量的控制和药效相关机制的研究提供参考。

Objective To research Guanghuoxiang (Pogostemonis Herba, PH) in different producing areas and predict the main active ingredients of PH in the treatment of viral infection and gastric ulcer based on UPLC fingerprint, “five principles” of quality markers and network pharmacology. Methods The UPLC fingerprint of PH was established, and the common peaks of 14 batches were analyzed by SIMCA-P 14.1 analysis software, and the main difference components were selected by partial least squares-discrimination analysis (PLS-DA). Based on quality markers “five principles”, the differential components were preliminarily analyzed; The differential compositions, virus infection and gastric ulcer disease related targets were retrieved through the related database by network pharmacology. David 6.8 database was used for gene ontology (GO) function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of common targets, and a “component-target-disease-pathway” network was constructed to analyze the main active components and key targets of PH. Results The results of UPLC fingerprint and cluster analysis showed that there were some differences in PH between the two producing areas in Guangdong Province. Four different components were selected by PLS-DA, which were verbascoside, pogostone, campneoside I and crenatoside in order. According to the “five principles” of quality markers combined with the network pharmacological analysis results, the main active ingredients of PH were pogostone and verbascoside. Both of them could regulate TLR signaling pathway, ErbB, MAPK and other signaling pathways by ERBB2, EGFR, TLR4, AKT1 and TNF targets, and played antiviral and gastrointestinal regulation roles. Conclusion The UPLC fingerprint method established in this study was simple, the two active ingredients could be used as potential quality markers of PH, which provided reference for the research on quality control of PH and the mechanism related to drug efficacy.

R283.6

国家科技重大专项项目（2019ZX09201005-002-006）