[关键词]
[摘要]
目的 在生物方剂分析药理（bioanalytical pharmacology，BAP）策略的指导下，检测柴胡疏肝散水提物中具有抗抑郁作用的可吸收生物活性物质，为柴胡疏肝散抗抑郁疗效的质量标志物（quality marker，Q-Marker）评价提供依据。方法 运用液相色谱-串联质谱（LC-MS/MS）测定柴胡疏肝散水提取物中的28种成分；大鼠ig柴胡疏肝散水提取物，定性分析大鼠血浆中的化合物，筛选生物活性物质，探索具有抗抑郁作用的Q-Marker。结果 从柴胡疏肝散水提物中的28种成分中确定了吸收入血的22个成分，筛选出的10个生物活性物质具有与柴胡疏肝散类似的抗抑郁疗效。结论 芍药内酯苷、阿魏酸、柚皮苷、橙皮苷、水合橙皮内酯、甘草酸、柴胡皂苷A、川陈皮素、橘皮素、甘草次酸为柴胡疏肝散抗抑郁作用的Q-Marker。
[Key word]
[Abstract]
Objective To detect the antidepressant absorption active compounds in water extract of Chaihu Shugan Powder, and provide a basis for evaluation of quality marker (Q-Marker) of Chaihu Shugan Powder antidepressant effect under the guidance of bioethnopharmaceutical analytical pharmacology (BAP) strategy. Methods LC-MS/MS was used to determine the 28 compounds in water extract of Chaihu Shugan Powder. Rats were ig given Chaihu Shugan Powder, compounds in rat plasma were analyzed and biological activity substances were screened to explore Q-Marker with antidepressant effects. Results Among 28 components in water extract of Chaihu Shugan Powder, 22 components were determined to be absorbed into blood. A total of 10 biological activity substances had antidepressant effects similar to Chaihu Shugan Powder. Conclusion Paeoniflorin, ferulic acid, naringin, hesperidin, hydrated hesperidin, glycyrrhizic acid, saikosaponin A, lichenpin, hesperetin, and glycyrrhetinic acid are Q-Markers for antidepressant effect of Chaihu Shugan Powder.
[中图分类号]
R285.5
[基金项目]
国家自然科学基金资助项目（81573797）
